biological source
rat pancreas
packaging
tube of 5 μg 94022431-DNA-5UG, pkg of vial of cells 94022431-1VL
growth mode
Adherent
karyotype
Not specified
morphology
Polygonal
products
Expresses CFTR protein
receptors
Lacks CCK-A receptors
technique(s)
cell culture | mammalian: suitable
relevant disease(s)
cancer
shipped in
dry ice
storage temp.
−196°C
Application
Study of pancreatic carcinomas, differentiation of acinar/ductal cells.
Biochem/physiol Actions
DSL6A/C1 were derived from a transplantable acinar cell carcinoma (DSL-6) that had been established from a primary carcinoma of the pancreas in an azaserine treated Lewis rat. The expression of the ductal cell marker OC.2 was shown with a sub-population expressing ductal marker OV-6, CK-7 and CK-19. DSL6A/C1 cells are reported to be tumorigenic in Lewis rats and produce solid tumours composed of duct like structures surrounded by dense fibrous tissue. The cell line lost structural and immunohistochemical acinar cell markers while acquiring duct cell markers during culture and re-grafting.
Rat pancreatic carcinoma
Preparation Note
Split sub-confluent cultures (70-80%) 1:2 to 1:3 i.e. seeding at 3-5x10,000 cells/cm2 using 0.25% trypsin or trypsin/EDTA; 5% CO2; 37°C.
Waymouth′s MB 752/1 + 2mM Glutamine + 10% Foetal Bovine Serum (FBS) (Heat Inactivated).
Other Notes
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法规信息
低风险生物材料
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相关内容
ECACC pancreatic cancer cell lines for various applications including drug response studies.
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