JY human cell line
IHW Number: 9287, NOTE: Both the cell line and DNA from the cell line may be available for this product. Please choose -1VL or VIAL for cells, or -DNA-5UG for DNA., 94022533
Synonym(s):
JY-S
biological source
human blood
packaging
tube of 5 μg 94022533-DNA-5UG
pkg of vial of cells 94022533-1VL
ethnicity
Amish
gender
male
growth mode
Suspension
karyotype
Not specified
morphology
Lymphoblastoid
technique(s)
cell culture | mammalian: suitable
shipped in
dry ice
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General description
Consanguineous: No
Homozygous: No
Biochem/physiol Actions
A: 201
Cw: 702
B: 702
HLA DQA1: 3011
HLA DPB1: 201
Cw: 702
B: 702
HLA DQA1: 3011
HLA DPB1: 201
This cell line is an Epstein-Barr Virus (EBV) transformed lymphoblastoid line and is part of the Human Leukocyte Antigen (HLA) Typed Collection maintained by the European Collection of Cell Cultures (ECACC). HLA-Typing Data is available for this cell line.
Preparation Note
RPMI 1640 (R0883) + 2mM Glutamine (G7513) + 20% Foetal Bovine Serum (FBS) (F2442). Once a growing culture is established the FBS concentration can be reduced to 10%.
When resuscitating from frozen, cells should be seeded at 0.5 x 106 cells/ml in the culture medium. The culture should establish log phase growth after 3-5 days. When growing well dilute every 1-3 days to 3 x 105 cells/ml. Maintain cultures between 3-9 x 105 cells/ml for maximum growth; 5% CO2; 37°C. Cells form floating aggregates of varying size.
Other Notes
Additional freight & handling charges may be applicable for Asia-Pacific shipments. Please check with your local Customer Service representative for more information.
HLA A: 2
HLA C: 7
HLA B: 7
Bw4/bw6: 6
HLA DR: 4 13
HLA DR52: 52
HLA DR53: 53
HLA DQ: 1 3
HLA C: 7
HLA B: 7
Bw4/bw6: 6
HLA DR: 4 13
HLA DR52: 52
HLA DR53: 53
HLA DQ: 1 3
Disclaimer
This cell line has restrictions and can only be supplied to academic and not-for-profit organisations. Nucleic acids can be supplied to all researchers including those from commercial organisations.
Regulatory Information
监管及禁止进口产品
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