SH-SY5Y Cell Line human
NOTE: Both the cell line and DNA from the cell line may be available for this product. Please choose -1VL or VIAL for cells, or -DNA-5UG for DNA, 94030304, human nerve, Neuroblast
Synonym(s):
SHSY5Y Cells, SK-SH-SY5Y Cells, SY5Y Cells
biological source
human nerve
packaging
tube of 5 μg 94030304-DNA-5UG
pkg of vial of cells 94030304-1VL
growth mode
Adherent
karyotype
Not specified
morphology
Neuroblast
products
Not specified
receptors
Not specified
technique(s)
cell culture | mammalian: suitable
relevant disease(s)
metastasis
shipped in
dry ice
storage temp.
−196°C
Application
SH-SY5Y cell line human is used for neurotransmitter studies
SH-SY5Y cell line human has been used to:
SH-SY5Y cell line human has been used to:
- study the iron-mediated toxicity of amyloid β peptide
- check the significance of anionic luminescent conjugated polythiophene (LCP) polythiophene acetic acid (PTAA) in vital staining of cells
- study the effect of haloperidol on sigma-1 receptors in guinea pig brain and human neuroblastoma cells
Biochem/physiol Actions
Human neuroblastoma
SH-SY5Y is a thrice-cloned sub-line of bone marrow biopsy-derived line SK-N-SH (Sigma catalogue no. 86012802). SH-SY-5Y has dopamine-β-hydroxylase activity and can convert glutamate to the neurotransmitter GABA. Will form tumours in nude mice in approximately 3-4 weeks. The loss of neuronal characteristics has been described with increasing passage numbers. Therefore it is recommended to verify specific characteristics such as noradrenalin uptake or neuronal markers routinely.
STR-PCR Data: Amelogenin: X
CSF1PO: 11
D13S317: 11
D16S539: 8,13
D5S818: 12
D7S820: 7,10
THO1: 7,10
TPOX: 8,11
vWA: 14,18
CSF1PO: 11
D13S317: 11
D16S539: 8,13
D5S818: 12
D7S820: 7,10
THO1: 7,10
TPOX: 8,11
vWA: 14,18
Packaging
NOTE: Both the cell line and DNA from the cell line may be available for this product. Please choose -1VL or VIAL for cells, or -DNA-5UG for DNA.
Preparation Note
Ham′s F12:EMEM (EBSS) (1:1) + 2mM Glutamine + 1% Non Essential Amino AcidsNEAA + 15% Foetal Bovine Serum FBS / FCS.
Split sub-confluent cultures (70-80%) 1:10 to 1:100 i.e. seeding at 1x1,000-1x10,000 cells/cm2; using 0.25% Trypsin or Trypsin/EDTA, 5% CO2; 37°C. Cells may reattach slowly and may remain in suspension for several days. The loss of neuronal characteristics has been described with increasing passage numbers. Therefore it is recommended to verify specific characteristics such as noradrenalin uptake or neuronal markers routinely.
Other Notes
- DNA is stored at -70 °C
- RNA is stored at -70 °C
- Cell line is stored at -196 °C
Additional freight & handling charges may be applicable for Asia-Pacific shipments. Please check with your local Customer Service representative for more information.
Regulatory Information
高风险级别生物产品--人源产品
动植物来源培养基
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