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文件

M7145

Sigma-Aldrich

MEM 非必需氨基酸溶液 (100×)

without L-glutamine, liquid, sterile-filtered, BioReagent, suitable for cell culture

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别名:
MEM NEAA
eCl@ss:
32160406
NACRES:
NA.75

质量水平

200

无菌性

sterile-filtered

产品线

BioReagent

形式

liquid

技术

cell culture | mammalian: suitable

杂质

endotoxin, tested

储存温度

2-8°C

相关类别

一般描述

Eagle′s基本必需培养基(MEM)是一种合成培养基，由平衡盐溶液、氨基酸和维生素组成。这些组份为培养细胞生长的必需组份。MEM可添加非必需氨基酸，而制备MEM非必需氨基酸溶液。非必需氨基酸包括L-丙氨酸、L-天冬酰胺、L-天冬氨酸、L-甘氨酸、L-丝氨酸、L-脯氨酸和L-谷氨酸。

应用

向培养基中添加氨基酸补充剂不仅可以刺激细胞生长还可以延长培养细胞的活力。MEM非必需氨基酸溶液的补充物，可增加营养成分并减少 体外细胞的生物合成负担。
MEM非必需氨基酸溶液已可用于维持哺乳动物细胞的Dulbecco′s基本必需培养基。它已被添加到卵母细胞成熟培养基中。

生化/生理作用

杂交瘤培养研究表明，Eagle8242;s基本培养基(MEM)添加非必需氨基酸可提高杂交瘤细胞在死亡阶段的膜稳定性。这也会导致死亡阶段抗体分泌增加，乳酸浓度下降。

其他说明

2024 CiteAb Award Winner for Supplier Succeeding in Parkinson′s Research

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储存分类代码

10 - Combustible liquids

WGK

WGK 3

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  1. Which document(s) contains shelf-life or expiration date information for a given product?

    If available for a given product, the recommended re-test date or the expiration date can be found on the Certificate of Analysis.

  2. How do I get lot-specific information or a Certificate of Analysis?

    The lot specific COA document can be found by entering the lot number above under the "Documents" section.

  3. How do I find price and availability?

    There are several ways to find pricing and availability for our products. Once you log onto our website, you will find the price and availability displayed on the product detail page. You can contact any of our Customer Sales and Service offices to receive a quote.  USA customers:  1-800-325-3010 or view local office numbers.

  4. What is the Department of Transportation shipping information for this product?

    Transportation information can be found in Section 14 of the product's (M)SDS.To access the shipping information for this material, use the link on the product detail page for the product. 

  5. Can I store Product M7145, MEM Non-essential Amino Acids, 100X solution at -20°C?

    Product M7145 is a 100X solution of non-essential amino acids that should be stored at 2-8 °C for maximum stability. If the product is properly stored and handled, we guarantee the product for one year after it is received. We have not tested the stability of the product at -20°C for long term storage, so we do not recommend aliquoting and freezing the product. It is likely that storage at -20°C will cause precipitation of some of the amino acids upon thawing. If precipitation occurs, the product should not be used.

  6. My question is not addressed here, how can I contact Technical Service for assistance?

    Ask a Scientist here.

Influence of non-essential amino acids on apoptotic and necrotic death of mouse hybridoma cells in batch cultures
Fassnacht D
Biotechnology Letters, 19(1), 35-38 (1997)
Cell Culture
Jakoby WB
Methods in Enzymology, 58 (1979)
Excessive concentration of glucose during in vitro maturation impairs the developmental competence of bovine oocytes after in vitro fertilization: relevance to intracellular reactive oxygen species and glutathione contents.
Hashimoto S et al.
Molecular Reproduction and Development, 56, 520-520 (2000)
Ayako Suzuki et al.
Genome biology, 16, 66-66 (2015-04-19)
To understand the heterogeneous behaviors of individual cancer cells, it is essential to investigate gene expression levels as well as their divergence between different individual cells. Recent advances in next-generation sequencing-related technologies have enabled us to conduct a single-cell RNA-Seq
Janis A Müller et al.
Medical microbiology and immunology, 206(2), 175-185 (2017-02-09)
Zika virus (ZIKV) is an emerging pathogen that causes congenital infections which may result in birth defects, such as microcephaly. Currently, no approved treatment or vaccination is available. ZIKV can be readily detected in cell culture where virally infected cells
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