biological source
human lung
packaging
tube of 5 μg 94060303-DNA-5UG
pkg of vial of cells 94060303-1VL
growth mode
Adherent
karyotype
Not specified
morphology
Epithelial
products
Synthesise the peptide neuromedin B (NMB) (0.1 pmol/mg protein).
receptors
Not specified
technique(s)
cell culture | mammalian: suitable
relevant disease(s)
cancer
shipped in
dry ice
storage temp.
−196°C
Application
Synthesis of NMB.
Biochem/physiol Actions
A non-small cell carcinoma of the lung from a 65 year old Caucasian female taken, prior to treatment. The cells express easily detectable levels of p53 mRNA compared to levels found in normal lung. The cells are not able to synthesise gastrin releasing peptide (GRP). NCI-H727 is the best differentiated available bronchial carcinoid cell line. Any proposed commercial use of these cells or products must first be negotiated with the University of Texas. More details available upon request.
Human lung non-small cell carcinoma
STR-PCR Data: Amelogenin: X
CSF1PO: 11,12
D13S317: 11
D16S539: 11,13
D5S818: 11,12
D7S820: 8,10
THO1: 8
TPOX: 8
vWA: 14,15
CSF1PO: 11,12
D13S317: 11
D16S539: 11,13
D5S818: 11,12
D7S820: 8,10
THO1: 8
TPOX: 8
vWA: 14,15
Preparation Note
RPMI 1640 + 2mM Glutamine + 10% Foetal Bovine Serum (FBS).
Split sub-confluent cultures (70-80%) 1:3 to 1:6 i.e. seeding at 2-4x10,000 cells/cm2 using 0.25% trypsin or trypsin/EDTA; 5% CO2; 37°C.
Other Notes
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