生物来源
human lung
包装
tube of 5 μg 94060303-DNA-5UG
pkg of vial of cells 94060303-1VL
生长模式
Adherent
核型
Not specified
形态学
Epithelial
产品
Synthesise the peptide neuromedin B (NMB) (0.1 pmol/mg protein).
受体
Not specified
技术
cell culture | mammalian: suitable
相关疾病
cancer
运输
dry ice
储存温度
−196°C
应用
Synthesis of NMB.
生化/生理作用
A non-small cell carcinoma of the lung from a 65 year old Caucasian female taken, prior to treatment. The cells express easily detectable levels of p53 mRNA compared to levels found in normal lung. The cells are not able to synthesise gastrin releasing peptide (GRP). NCI-H727 is the best differentiated available bronchial carcinoid cell line. Any proposed commercial use of these cells or products must first be negotiated with the University of Texas. More details available upon request.
Human lung non-small cell carcinoma
STR-PCR Data: Amelogenin: X
CSF1PO: 11,12
D13S317: 11
D16S539: 11,13
D5S818: 11,12
D7S820: 8,10
THO1: 8
TPOX: 8
vWA: 14,15
CSF1PO: 11,12
D13S317: 11
D16S539: 11,13
D5S818: 11,12
D7S820: 8,10
THO1: 8
TPOX: 8
vWA: 14,15
制备说明
RPMI 1640 + 2mM Glutamine + 10% Foetal Bovine Serum (FBS).
Split sub-confluent cultures (70-80%) 1:3 to 1:6 i.e. seeding at 2-4x10,000 cells/cm2 using 0.25% trypsin or trypsin/EDTA; 5% CO2; 37°C.
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