biological source
dog blood
packaging
tube of 5 μg 94062922-DNA-5UG, pkg of vial of cells 94062922-1VL
growth mode
Suspension
karyotype
Not specified
morphology
Macrophage
products
Not specified
receptors
FC-gamma receptors
technique(s)
cell culture | mammalian: suitable
relevant disease(s)
cancer
shipped in
dry ice
storage temp.
−196°C
Biochem/physiol Actions
Canine Monocyte-macrophage
Derived from a ten year old male golden retriever with malignant histiocytosis. The cells have a macrophage-like morphology and are able to phagocytose latex particles. They are positive for Fc-gamma receptors and are negative for Fc-mu and C3b receptors. DH82 cells do not produce IL-1.
Preparation Note
EMEM (EBSS) + 2mM Glutamine + 1% Non Essential Amino Acids (NEAA) + 15% Foetal Bovine Serum (FBS) (Heat Inactivated).
Maintain cultures at 3-9x100,000 cells/ml; 5% CO2; 37°C. Cells are prone to sticking and can be removed with 0.02% EDTA.
Other Notes
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This cell line is part of the European Collection of Authenticated Cell Cultures (ECACC), an international repository managed by the United Kingdom Health Security Agency (UKHSA). No licensing agreement is required when either this cell line or the DNA extracted from it are used for internal research purposes only. Any other use of these products is prohibited without the express written permission of UKHSA. Inquiries regarding authorized use of this cell line or its genomic DNA may be directed to culturecollections@ukhsa.gov.uk.