biological source
dog blood
packaging
tube of 5 μg 94062922-DNA-5UG, pkg of vial of cells 94062922-1VL
growth mode
Suspension
karyotype
Not specified
morphology
Macrophage
products
Not specified
receptors
FC-gamma receptors
technique(s)
cell culture | mammalian: suitable
relevant disease(s)
cancer
shipped in
dry ice
storage temp.
−196°C
Biochem/physiol Actions
Canine Monocyte-macrophage
Derived from a ten year old male golden retriever with malignant histiocytosis. The cells have a macrophage-like morphology and are able to phagocytose latex particles. They are positive for Fc-gamma receptors and are negative for Fc-mu and C3b receptors. DH82 cells do not produce IL-1.
Preparation Note
EMEM (EBSS) + 2mM Glutamine + 1% Non Essential Amino Acids (NEAA) + 15% Foetal Bovine Serum (FBS) (Heat Inactivated).
Maintain cultures at 3-9x100,000 cells/ml; 5% CO2; 37°C. Cells are prone to sticking and can be removed with 0.02% EDTA.
Other Notes
Additional freight & handling charges may be applicable for Asia-Pacific shipments. Please check with your local Customer Service representative for more information.
法规信息
低风险生物材料
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