KYSE-270
NOTE: Both the cell line and DNA from the cell line may be available for this product. Please choose -1VL or VIAL for cells, or -DNA-5UG for DNA, 94072021, human esophagus, Epithelial
Synonym(s):
KY-270 Cells, KYSE270 Cells
biological source
human esophagus
packaging
tube of 5 μg 94072021-DNA-5UG
pkg of vial of cells 94072021-1VL
growth mode
Adherent
karyotype
Hypotriploid with 16% polyploidy
morphology
Epithelial
products
Not specified
receptors
Not specified
technique(s)
cell culture | mammalian: suitable
relevant disease(s)
cancer
shipped in
dry ice
storage temp.
−196°C
Biochem/physiol Actions
Established from the well differentiated invasive oesophageal squamous cell carcinoma resected from the middle intra-thoracic oesophagus of a 79-year-old Japanese man prior to treatment. This cell line has been mycoplasma eradicated at ECACC.
Human oesophageal squamous cell carcinoma
Preparation Note
RPMI 1640:HAMS F12 (1:1) + 2mM Glutamine + 2% Foetal Bovine Serum (FBS)
Split confluent cultures 1:5 every second day using trypsin/EDTA;5% CO2; 37°C. Doubling time: 24 hours. Recommended seeding density: 2.5 x 10,000 cells/cm2.
Other Notes
Additional freight & handling charges may be applicable for Asia-Pacific shipments. Please check with your local Customer Service representative for more information.
Regulatory Information
动植物来源培养基
高风险级别生物产品--人源产品
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