生物来源
human esophagus
包装
tube of 5 μg 94072021-DNA-5UG
pkg of vial of cells 94072021-1VL
生长模式
Adherent
核型
Hypotriploid with 16% polyploidy
形态学
Epithelial
产品
Not specified
受体
Not specified
技术
cell culture | mammalian: suitable
相关疾病
cancer
运输
dry ice
储存温度
−196°C
细胞系来源
Human oesophageal squamous cell carcinoma
细胞系描述
Established from the well differentiated invasive oesophageal squamous cell carcinoma resected from the middle intra-thoracic oesophagus of a 79-year-old Japanese man prior to treatment. This cell line has been mycoplasma eradicated at ECACC.
培养基
RPMI 1640:HAMS F12 (1:1) + 2mM Glutamine + 2% Foetal Bovine Serum (FBS)
传代培养常规
Split confluent cultures 1:5 every second day using trypsin/EDTA;5% CO2; 37°C. Doubling time: 24 hours. Recommended seeding density: 2.5 x 10,000 cells/cm2.
其他说明
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