biological source
hamster ovary
packaging
tube of 5 μg 940822109-DNA-5UG, pkg of vial of cells 940822109-1VL
growth mode
Adherent
karyotype
Not specified
morphology
Epithelial
products
Not specified
receptors
Not specified
technique(s)
cell culture | mammalian: suitable
shipped in
dry ice
storage temp.
−196°C
Application
This cell line can be used to study m1 mAChR in cells devoid of other muscarinic receptor subtypes.
Biochem/physiol Actions
Derived in 1990 by transfecting Chinese Hamster ovary CHO-K1 cells with the gene encoding rat m1 muscarinic acetylcholine receptor (mAChR) in the mammalian expression vector, pSVL. These cells were then co-transfected with pMSVNeo and selected for growth in selection medium containing 500μg/ml G418. The cells are reported to express 800 fmol of receptor/mg of membrane protein.
Hamster Chinese ovary (transfected with rat m1 mAChR encoding gene).
Preparation Note
Ham′s F12 + 2mM Glutamine + 50-100μg/ml G418 + 10% Foetal Bovine Serum (FBS).
Split sub-confluent cultures (70-80%) 1:4 to 1:8 i.e. seeding at 1-2x10,000 cells/cm2 using 0.25% trypsin or trypsin/EDTA; 5% CO2; 37°C.
Other Notes
Additional freight & handling charges may be applicable for Asia-Pacific shipments. Please check with your local Customer Service representative for more information.
This cell line is part of the European Collection of Authenticated Cell Cultures (ECACC), an international repository managed by the United Kingdom Health Security Agency (UKHSA). No licensing agreement is required when either this cell line or the DNA extracted from it are used for internal research purposes only. Any other use of these products is prohibited without the express written permission of UKHSA. Inquiries regarding authorized use of this cell line or its genomic DNA may be directed to culturecollections@ukhsa.gov.uk.