biological source
hamster ovary
packaging
tube of 5 μg 940822109-DNA-5UG, pkg of vial of cells 940822109-1VL
growth mode
Adherent
karyotype
Not specified
morphology
Epithelial
products
Not specified
receptors
Not specified
technique(s)
cell culture | mammalian: suitable
shipped in
dry ice
storage temp.
−196°C
Application
This cell line can be used to study m1 mAChR in cells devoid of other muscarinic receptor subtypes.
Biochem/physiol Actions
Derived in 1990 by transfecting Chinese Hamster ovary CHO-K1 cells with the gene encoding rat m1 muscarinic acetylcholine receptor (mAChR) in the mammalian expression vector, pSVL. These cells were then co-transfected with pMSVNeo and selected for growth in selection medium containing 500μg/ml G418. The cells are reported to express 800 fmol of receptor/mg of membrane protein.
Hamster Chinese ovary (transfected with rat m1 mAChR encoding gene).
Preparation Note
Ham′s F12 + 2mM Glutamine + 50-100μg/ml G418 + 10% Foetal Bovine Serum (FBS).
Split sub-confluent cultures (70-80%) 1:4 to 1:8 i.e. seeding at 1-2x10,000 cells/cm2 using 0.25% trypsin or trypsin/EDTA; 5% CO2; 37°C.
Other Notes
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法规信息
低风险生物材料
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