Sign In to View Organizational & Contract Pricing.
Select a Size
About This Item
UNSPSC Code:
41106514
Biological source:
human blood
Technique(s):
cell culture | mammalian: suitable
Ethnicity:
Not specified
Gender:
not specified
Growth mode:
Suspension
Morphology:
Lymphoblastoid
biological source
human blood
packaging
tube of 5 μg 94082249-DNA-5UG, pkg of vial of cells 94082249-1VL
ethnicity
Not specified
gender
not specified
growth mode
Suspension
karyotype
Not specified
morphology
Lymphoblastoid
technique(s)
cell culture | mammalian: suitable
shipped in
dry ice
General description
Consanguineous: No
Homozygous: No
Biochem/physiol Actions
HLA DRB1: 0301 07
HLA DRB3: 101
HLA DRB4: 101
HLA DQAP: 2.1 4.1
HLA DQA1: 0501 0201
HLA DQB1: 201
HLA DPB1: 0401 0402
HLA DRB3: 101
HLA DRB4: 101
HLA DQAP: 2.1 4.1
HLA DQA1: 0501 0201
HLA DQB1: 201
HLA DPB1: 0401 0402
This cell line is an Epstein-Barr Virus (EBV) transformed lymphoblastoid line and is part of the Human Leukocyte Antigen (HLA) Typed Collection maintained by the European Collection of Cell Cultures (ECACC). HLA-Typing Data is available for this cell line.
Preparation Note
RPMI 1640 (R0883) + 2mM Glutamine (G7513) + 20% Foetal Bovine Serum (FBS) (F2442). Once a growing culture is established the FBS concentration can be reduced to 10%.
Maintain cultures between 3x105 - 2x106 cells/ml, 5% CO2, 37 °C. Upon resuscitation remove cryoprotectant - centrifuge at 300 x g for 5 minutes and re-suspend the cell pellet in fresh culture medium.
Other Notes
Cultures from HPA Culture Collections and supplied by Sigma are for research purposes only. Enquiries regarding the commercial use of a cell line are referred to the depositor of the cell line. Some cell lines have additional special release conditions such as the requirement for a material transfer agreement to be completed by the potential recipient prior to the supply of the cell line. Please view the Terms & Conditions of Supply for more information.
HLA A: 1 32
HLA C: 7
HLA B: 8 14
Bw4/bw6: 6
HLA C: 7
HLA B: 8 14
Bw4/bw6: 6
Regulatory Information
新产品
This item has
Choose from one of the most recent versions:
Already Own This Product?
Find documentation for the products that you have recently purchased in the Document Library.
Our team of scientists has experience in all areas of research including Life Science, Material Science, Chemical Synthesis, Chromatography, Analytical and many others.
Contact Technical Service