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VA-ES-BJ

NOTE: Both the cell line and DNA from the cell line may be available for this product. Please choose -1VL or VIAL for cells, or -DNA-5UG for DNA.

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About This Item

UNSPSC Code:
41106514
Biological source:
human skin (bone marrow metastasis)
Relevant disease(s):
metastasis
Growth mode:
Adherent
Karyotype:
66-68 Aneuploidic, pentasomy #7, multiple trisomy
Morphology:
Epithelial

Key Documents

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biological source

human skin (bone marrow metastasis)

packaging

tube of 5 μg 94092303-DNA-5UG, pkg of vial of cells 94092303-1VL

growth mode

Adherent

karyotype

66-68 Aneuploidic, pentasomy #7, multiple trisomy

morphology

Epithelial

products

Granulocyte Macrophage Colony Stimulating Factor (GM-CSF)

receptors

Granulocyte Macrophage Colony Stimulating Factor (GM-CSF), AE, AE3, Fas, epithelial membrane antigen (EMA), Vimentin, P53

technique(s)

cell culture | mammalian: suitable

relevant disease(s)

metastasis

shipped in

dry ice

storage temp.

−196°C

Related Categories

Application

Study of tumours. Tumourigenic in nude mice.

Biochem/physiol Actions

Human skin epithelioid sarcoma
Spontaneously transformed cells from a male patient with epithelioid sarcoma derived from bone marrow metastasis. Cells grow very rapidly and secrete GM-CSF. They have been shown to express AE-1 and AE-3 cytokeratins, epithelial membrane antigen, p53, human Fas and vimentin. VA-ES-BJ cells are sensitive to oxidising agents, busulfaxamine and protoporphyrin IX. Epithelial, spindle and giant cells.

Preparation Note

DMEM + 2mM Glutamine + 1% Non Essential Amino Acids (NEAA) + 10% Foetal Bovine Serum (FBS).
Split sub-confluent cultures (70-80%) 1:3 to 1:6 i.e. seeding at 2-4x10,000 cells/cm2 using 0.25% trypsin/EDTA; 5% CO2; 37°C.

Other Notes

Additional freight & handling charges may be applicable for Asia-Pacific shipments. Please check with your local Customer Service representative for more information.

Regulatory Information

常规特殊物品
低风险生物材料
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