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关于此项目
UNSPSC Code:
41106514
Biological source:
human skin (bone marrow metastasis)
Relevant disease(s):
metastasis
Growth mode:
Adherent
Karyotype:
66-68 Aneuploidic, pentasomy #7, multiple trisomy
Morphology:
Epithelial
biological source
human skin (bone marrow metastasis)
packaging
tube of 5 μg 94092303-DNA-5UG, pkg of vial of cells 94092303-1VL
growth mode
Adherent
karyotype
66-68 Aneuploidic, pentasomy #7, multiple trisomy
morphology
Epithelial
products
Granulocyte Macrophage Colony Stimulating Factor (GM-CSF)
receptors
Granulocyte Macrophage Colony Stimulating Factor (GM-CSF), AE, AE3, Fas, epithelial membrane antigen (EMA), Vimentin, P53
technique(s)
cell culture | mammalian: suitable
relevant disease(s)
metastasis
shipped in
dry ice
storage temp.
−196°C
Application
Study of tumours. Tumourigenic in nude mice.
Biochem/physiol Actions
Human skin epithelioid sarcoma
Spontaneously transformed cells from a male patient with epithelioid sarcoma derived from bone marrow metastasis. Cells grow very rapidly and secrete GM-CSF. They have been shown to express AE-1 and AE-3 cytokeratins, epithelial membrane antigen, p53, human Fas and vimentin. VA-ES-BJ cells are sensitive to oxidising agents, busulfaxamine and protoporphyrin IX. Epithelial, spindle and giant cells.
Preparation Note
DMEM + 2mM Glutamine + 1% Non Essential Amino Acids (NEAA) + 10% Foetal Bovine Serum (FBS).
Split sub-confluent cultures (70-80%) 1:3 to 1:6 i.e. seeding at 2-4x10,000 cells/cm2 using 0.25% trypsin/EDTA; 5% CO2; 37°C.
Other Notes
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This cell line is part of the European Collection of Authenticated Cell Cultures (ECACC), an international repository managed by the United Kingdom Health Security Agency (UKHSA). No licensing agreement is required when either this cell line or the DNA extracted from it are used for internal research purposes only. Any other use of these products is prohibited without the express written permission of UKHSA. Inquiries regarding authorized use of this cell line or its genomic DNA may be directed to culturecollections@ukhsa.gov.uk.