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UNSPSC Code:
41106514
Biological source:
human skin (bone marrow metastasis)
Relevant disease(s):
metastasis
Growth mode:
Adherent
Karyotype:
66-68 Aneuploidic, pentasomy #7, multiple trisomy
Morphology:
Epithelial
biological source
human skin (bone marrow metastasis)
packaging
tube of 5 μg 94092303-DNA-5UG, pkg of vial of cells 94092303-1VL
growth mode
Adherent
karyotype
66-68 Aneuploidic, pentasomy #7, multiple trisomy
morphology
Epithelial
products
Granulocyte Macrophage Colony Stimulating Factor (GM-CSF)
receptors
Granulocyte Macrophage Colony Stimulating Factor (GM-CSF), AE, AE3, Fas, epithelial membrane antigen (EMA), Vimentin, P53
technique(s)
cell culture | mammalian: suitable
relevant disease(s)
metastasis
shipped in
dry ice
storage temp.
−196°C
Application
Study of tumours. Tumourigenic in nude mice.
Biochem/physiol Actions
Human skin epithelioid sarcoma
Spontaneously transformed cells from a male patient with epithelioid sarcoma derived from bone marrow metastasis. Cells grow very rapidly and secrete GM-CSF. They have been shown to express AE-1 and AE-3 cytokeratins, epithelial membrane antigen, p53, human Fas and vimentin. VA-ES-BJ cells are sensitive to oxidising agents, busulfaxamine and protoporphyrin IX. Epithelial, spindle and giant cells.
Preparation Note
DMEM + 2mM Glutamine + 1% Non Essential Amino Acids (NEAA) + 10% Foetal Bovine Serum (FBS).
Split sub-confluent cultures (70-80%) 1:3 to 1:6 i.e. seeding at 2-4x10,000 cells/cm2 using 0.25% trypsin/EDTA; 5% CO2; 37°C.
Other Notes
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法规信息
常规特殊物品
低风险生物材料
此项目有
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