biological source
rat liver
packaging
tube of 5 μg 94101907-DNA-5UG, pkg of vial of cells 94101907-1VL
growth mode
Adherent
karyotype
Hyperdiploid, 51 chromosomes
morphology
Epithelial
products
Albumin and hepatocyte markers
receptors
Not specified
technique(s)
cell culture | mammalian: suitable
relevant disease(s)
cancer
shipped in
dry ice
storage temp.
−196°C
Application
Gene expression studies.
Biochem/physiol Actions
C2-Rev 7 is a revertant of the C2 variants, selected by virtue of its capacity to grow in glucose free medium (containing dialysed serum) where survival requires activity of the liver-specific gluconeogenic pathway. Expression of the entire set of liver specific functions is restored. Albumin and hepatocyte markers present.
Rat hepatoma [HGPRT-]
Preparation Note
Coon′s modified Ham′s F12 (or Kaign′s modified Ham′s F12 + 45mg/L ascorbic acid + 18mg/L Inositol) + 2mM Glutamine + 5% Foetal Bovine Serum (FBS).
Split semi-confluent cultures (70%) 1:10 i.e. seeding at 9x1000 cells/cm2 using 0.25% trypsin or trypsin/EDTA; 5% CO2; 37°C. Saturation density is reached at 15x1,000,000 cells/10cm2 dish.
Other Notes
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This cell line is part of the European Collection of Authenticated Cell Cultures (ECACC), an international repository managed by the United Kingdom Health Security Agency (UKHSA). No licensing agreement is required when either this cell line or the DNA extracted from it are used for internal research purposes only. Any other use of these products is prohibited without the express written permission of UKHSA. Inquiries regarding authorized use of this cell line or its genomic DNA may be directed to culturecollections@ukhsa.gov.uk.