biological source
rat liver
packaging
tube of 5 μg 94101907-DNA-5UG, pkg of vial of cells 94101907-1VL
growth mode
Adherent
karyotype
Hyperdiploid, 51 chromosomes
morphology
Epithelial
products
Albumin and hepatocyte markers
receptors
Not specified
technique(s)
cell culture | mammalian: suitable
relevant disease(s)
cancer
shipped in
dry ice
storage temp.
−196°C
Application
Gene expression studies.
Biochem/physiol Actions
C2-Rev 7 is a revertant of the C2 variants, selected by virtue of its capacity to grow in glucose free medium (containing dialysed serum) where survival requires activity of the liver-specific gluconeogenic pathway. Expression of the entire set of liver specific functions is restored. Albumin and hepatocyte markers present.
Rat hepatoma [HGPRT-]
Preparation Note
Coon′s modified Ham′s F12 (or Kaign′s modified Ham′s F12 + 45mg/L ascorbic acid + 18mg/L Inositol) + 2mM Glutamine + 5% Foetal Bovine Serum (FBS).
Split semi-confluent cultures (70%) 1:10 i.e. seeding at 9x1000 cells/cm2 using 0.25% trypsin or trypsin/EDTA; 5% CO2; 37°C. Saturation density is reached at 15x1,000,000 cells/10cm2 dish.
Other Notes
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法规信息
低风险生物材料
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