MAT-Lu

Carcinogenesis studies, cytogenetic analysis

MAT-Lu is one of a large series of cell lines of transplantable prostatic tumours originating from the spontaneous Dunning R-3327 rat prostatic cancer. The MAT-Lu cell line has been derived from a Metastatic-Anaplastic Tumour metastasising to the lungs (=MAT-Lu) and is androgen-independent.

Rat dorsal prostate adenocarcinoma

RPMI 1640 + 2mM Glutamine + 250nM Dexamethasone (DXMT) + 10% Foetal Bovine Serum (FBS).

Split sub-confluent cultures (70-80%) 1:10 to 1:20 i.e. seeding at 2-4x1000 cells/cm² using 0.25% trypsin/EDTA; 5% CO₂; 37°C.

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This cell line is part of the European Collection of Authenticated Cell Cultures (ECACC), an international repository managed by the United Kingdom Health Security Agency (UKHSA). No licensing agreement is required when either this cell line or the DNA extracted from it are used for internal research purposes only. Any other use of these products is prohibited without the express written permission of UKHSA. Inquiries regarding authorized use of this cell line or its genomic DNA may be directed to culturecollections@ukhsa.gov.uk.