Sign In to View Organizational & Contract Pricing.
Select a Size
About This Item
UNSPSC Code:
41106514
Biological source:
human nerve
Relevant disease(s):
cancer
Growth mode:
Adherent
Karyotype:
Not specified
Morphology:
Fusiform
biological source
human nerve
packaging
tube of 5 μg 95011816-DNA-5UG, pkg of vial of cells 95011816-1VL
growth mode
Adherent
karyotype
Not specified
morphology
Fusiform
products
Not specified
receptors
Not specified
technique(s)
cell culture | mammalian: suitable
relevant disease(s)
cancer
shipped in
dry ice
storage temp.
−196°C
Application
Study of neuroblastoma
Biochem/physiol Actions
Human Caucasian neuroblastoma
The cell line BE(2)-M17 was isolated from SK-N-BE(2) (Sigma Catalogue number. 95011815) by limiting dilution. The cells show a population doubling time of 20-24 hours, grow in multi-layers but start to aggregate and float as the culture ages. The neuroblastic cells express properties of neuroadrenergic neurones and multiple, moderately long cell processes. BE(2)-M17 showed tyrosine hydroxylase and dopamine-β-hydroxylase activity and specific uptake of norepinephrine (noradrenalin). Expression of intermediate filaments was detected as well as neuron-specific enolase and moderate expression of vimentin. BE(2)-M17 are tumorigenic in nude mice.
Preparation Note
EMEM (EBSS):Ham′s F12 (1:1) + 2mM Glutamine + 1% Non Essential Amino Acids (NEAA) + 15% Foetal Bovine Serum (FBS) (Heat Inactivated).
Split sub-confluent cultures (70-80%) 1:8 to 1:20 i.e. seeding at 4x10,000 cells/cm2 using 0.1% trypsin or PBS only; 5% CO2; 37°C.
Other Notes
Additional freight & handling charges may be applicable for Asia-Pacific shipments. Please check with your local Customer Service representative for more information.
Regulatory Information
常规特殊物品
低风险生物材料
This item has
Choose from one of the most recent versions:
Already Own This Product?
Find documentation for the products that you have recently purchased in the Document Library.
Our team of scientists has experience in all areas of research including Life Science, Material Science, Chemical Synthesis, Chromatography, Analytical and many others.
Contact Technical Service