biological source
human nerve
packaging
tube of 5 μg 95011816-DNA-5UG, pkg of vial of cells 95011816-1VL
growth mode
Adherent
karyotype
Not specified
morphology
Fusiform
products
Not specified
receptors
Not specified
technique(s)
cell culture | mammalian: suitable
relevant disease(s)
cancer
shipped in
dry ice
storage temp.
−196°C
Application
Study of neuroblastoma
Biochem/physiol Actions
Human Caucasian neuroblastoma
The cell line BE(2)-M17 was isolated from SK-N-BE(2) (Sigma Catalogue number. 95011815) by limiting dilution. The cells show a population doubling time of 20-24 hours, grow in multi-layers but start to aggregate and float as the culture ages. The neuroblastic cells express properties of neuroadrenergic neurones and multiple, moderately long cell processes. BE(2)-M17 showed tyrosine hydroxylase and dopamine-β-hydroxylase activity and specific uptake of norepinephrine (noradrenalin). Expression of intermediate filaments was detected as well as neuron-specific enolase and moderate expression of vimentin. BE(2)-M17 are tumorigenic in nude mice.
Preparation Note
EMEM (EBSS):Ham′s F12 (1:1) + 2mM Glutamine + 1% Non Essential Amino Acids (NEAA) + 15% Foetal Bovine Serum (FBS) (Heat Inactivated).
Split sub-confluent cultures (70-80%) 1:8 to 1:20 i.e. seeding at 4x10,000 cells/cm2 using 0.1% trypsin or PBS only; 5% CO2; 37°C.
Other Notes
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法规信息
低风险生物材料
常规特殊物品
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