BE(2)-M17

Study of neuroblastoma

Human Caucasian neuroblastoma

The cell line BE(2)-M17 was isolated from SK-N-BE(2) (Sigma Catalogue number. 95011815) by limiting dilution. The cells show a population doubling time of 20-24 hours, grow in multi-layers but start to aggregate and float as the culture ages. The neuroblastic cells express properties of neuroadrenergic neurones and multiple, moderately long cell processes. BE(2)-M17 showed tyrosine hydroxylase and dopamine-ß-hydroxylase activity and specific uptake of norepinephrine (noradrenalin). Expression of intermediate filaments was detected as well as neuron-specific enolase and moderate expression of vimentin. BE(2)-M17 are tumorigenic in nude mice.

EMEM (EBSS):Ham′s F12 (1:1) + 2mM Glutamine + 1% Non Essential Amino Acids (NEAA) + 15% Foetal Bovine Serum (FBS) (Heat Inactivated).

Split sub-confluent cultures (70-80%) 1:8 to 1:20 i.e. seeding at 4x10,000 cells/cm² using 0.1% trypsin or PBS only; 5% CO₂; 37°C.

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