Sign In to View Organizational & Contract Pricing.
Select a Size
About This Item
eCl@ss:
32190102
UNSPSC Code:
41106514
Biological source:
human uterus
Relevant disease(s):
cancer
Growth mode:
Adherent
Karyotype:
Modal no. 47 (parent line MES-SA-45). Reported karyotype 47,XX,12qt,22q-t(5q,6p),-3,-4,-7,+t(3q?,7q),Mar1,+mar2,+Mar3
Morphology:
Epithelial
biological source
human uterus
packaging
tube of 5 μg 95051031-DNA-5UG, pkg of vial of cells 95051031-1VL
growth mode
Adherent
karyotype
Modal no. 47 (parent line MES-SA-45). Reported karyotype 47,XX,12qt,22q-t(5q,6p),-3,-4,-7,+t(3q?,7q),Mar1,+mar2,+Mar3
morphology
Epithelial
products
High levels of Mdr1 mRNA and P-glycoprotein
receptors
Not specified
technique(s)
cell culture | mammalian: suitable
relevant disease(s)
cancer
shipped in
dry ice
storage temp.
−196°C
Application
Tumour toxicity assays and multi-drug-resistant studies.
Biochem/physiol Actions
Human uterus sarcoma
The multi drug-resistant cell line MES-SA/Dx5 was derived from the human uterine sarcoma cell line MES-SA (Sigma Catalogue number. 95051030) which was originally obtained from a tumour from a 56-year-old Caucasian female at the time of hysterectomy. The Dx5 variant exhibits a 100-fold resistance to doxorubicin and has a reported doubling time of 30 hours. The two additional marker chromosomes indicate clonal selection during drug selection. MES-SA/Dx-5 cells exhibit marked cross-resistance to a number of chemotherapeutic agents (including daunorubicin, dactinomycin, vincristine, taxol, colchicine) and moderate cross-resistance to mitomycin C and melphalan. Cross resistance to bleomycin, cisplatin, carmustine, 5-fluorouracil or methotrexate was not observed.
Preparation Note
McCoy′s 5a + 2mM Glutamine + 10% Foetal Bovine Serum (FBS).
Split sub-confluent cultures (70-80%) 1:6 to 1:8 i.e. seeding at 1-3 x 10,000 cells/cm2 using 0.03% EDTA; 5% CO2; 37°C. Tap side of flask to dislodge cells.
Other Notes
Additional freight & handling charges may be applicable for Asia-Pacific shipments. Please check with your local Customer Service representative for more information.
Regulatory Information
低风险生物材料
常规特殊物品
This item has
Choose from one of the most recent versions:
Already Own This Product?
Find documentation for the products that you have recently purchased in the Document Library.
Our team of scientists has experience in all areas of research including Life Science, Material Science, Chemical Synthesis, Chromatography, Analytical and many others.
Contact Technical Service