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eCl@ss:
32190102
UNSPSC Code:
41106514
Biological source:
human uterus
Relevant disease(s):
cancer
Growth mode:
Adherent
Karyotype:
Modal no. 47 (parent line MES-SA-45). Reported karyotype 47,XX,12qt,22q-t(5q,6p),-3,-4,-7,+t(3q?,7q),Mar1,+mar2,+Mar3
Morphology:
Epithelial
biological source
human uterus
packaging
tube of 5 μg 95051031-DNA-5UG, pkg of vial of cells 95051031-1VL
growth mode
Adherent
karyotype
Modal no. 47 (parent line MES-SA-45). Reported karyotype 47,XX,12qt,22q-t(5q,6p),-3,-4,-7,+t(3q?,7q),Mar1,+mar2,+Mar3
morphology
Epithelial
products
High levels of Mdr1 mRNA and P-glycoprotein
receptors
Not specified
technique(s)
cell culture | mammalian: suitable
relevant disease(s)
cancer
shipped in
dry ice
storage temp.
−196°C
Application
Tumour toxicity assays and multi-drug-resistant studies.
Biochem/physiol Actions
Human uterus sarcoma
The multi drug-resistant cell line MES-SA/Dx5 was derived from the human uterine sarcoma cell line MES-SA (Sigma Catalogue number. 95051030) which was originally obtained from a tumour from a 56-year-old Caucasian female at the time of hysterectomy. The Dx5 variant exhibits a 100-fold resistance to doxorubicin and has a reported doubling time of 30 hours. The two additional marker chromosomes indicate clonal selection during drug selection. MES-SA/Dx-5 cells exhibit marked cross-resistance to a number of chemotherapeutic agents (including daunorubicin, dactinomycin, vincristine, taxol, colchicine) and moderate cross-resistance to mitomycin C and melphalan. Cross resistance to bleomycin, cisplatin, carmustine, 5-fluorouracil or methotrexate was not observed.
Preparation Note
McCoy′s 5a + 2mM Glutamine + 10% Foetal Bovine Serum (FBS).
Split sub-confluent cultures (70-80%) 1:6 to 1:8 i.e. seeding at 1-3 x 10,000 cells/cm2 using 0.03% EDTA; 5% CO2; 37°C. Tap side of flask to dislodge cells.
Other Notes
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法规信息
低风险生物材料
常规特殊物品
此项目有
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