MES-SA/Dx-5
NOTE: Both the cell line and DNA from the cell line may be available for this product. Please choose -1VL or VIAL for cells, or -DNA-5UG for DNA, 95051031, human uterus, Epithelial
生物来源
human uterus
包装
tube of 5 μg 95051031-DNA-5UG
pkg of vial of cells 95051031-1VL
生长模式
Adherent
核型
Modal no. 47 (parent line MES-SA-45). Reported karyotype 47,XX,12qt,22q-t(5q,6p),-3,-4,-7,+t(3q?,7q),Mar1,+mar2,+Mar3
形态学
Epithelial
产品
High levels of Mdr1 mRNA and P-glycoprotein
受体
Not specified
技术
cell culture | mammalian: suitable
相关疾病
cancer
运输
dry ice
储存温度
−196°C
应用
Tumour toxicity assays and multi-drug-resistant studies.
生化/生理作用
Human uterus sarcoma
The multi drug-resistant cell line MES-SA/Dx5 was derived from the human uterine sarcoma cell line MES-SA (Sigma Catalogue number. 95051030) which was originally obtained from a tumour from a 56-year-old Caucasian female at the time of hysterectomy. The Dx5 variant exhibits a 100-fold resistance to doxorubicin and has a reported doubling time of 30 hours. The two additional marker chromosomes indicate clonal selection during drug selection. MES-SA/Dx-5 cells exhibit marked cross-resistance to a number of chemotherapeutic agents (including daunorubicin, dactinomycin, vincristine, taxol, colchicine) and moderate cross-resistance to mitomycin C and melphalan. Cross resistance to bleomycin, cisplatin, carmustine, 5-fluorouracil or methotrexate was not observed.
制备说明
McCoy′s 5a + 2mM Glutamine + 10% Foetal Bovine Serum (FBS).
Split sub-confluent cultures (70-80%) 1:6 to 1:8 i.e. seeding at 1-3 x 10,000 cells/cm2 using 0.03% EDTA; 5% CO2; 37°C. Tap side of flask to dislodge cells.
其他说明
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