L8

Study of muscle differentiation, expression and analysis of muscle-specific proteins

Rat Skeletal muscle myoblast

The myogenic cell line L8 was isolated by D Yaffe in 1969 by selective serial passage of myoblasts from primary rat skeletal muscle cell cultures (new born, non-in-bred Wistar rats). Subsequently they were recloned by H Blau. L8 cells differentiate into cross-striated myotubes by fusion and express several muscle-specific proteins. The myoblast population will be depleted if cells are allowed to become confluent before sub-culturing. Therefore, sub-cultivation before confluency and periodical re-cloning is necessary. The preparation of a frozen stock at early passage is recommended.

DMEM : Medium 199 (4:1) + 2mM Glutamine + 1% CEE (Chick Embryo extract (Seralab)) + 10% Horse Serum (HS).

Split sub-confluent cultures (70-80%) 1:5 to 1:10 i.e. seeding at 5x1,000 cells to 1x10,000 cells/cm² using 0.25% trypsin or trypsin/EDTA; 5% CO₂; 37°C; sub-cultivate before confluency and reclone periodically.

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This cell line is part of the European Collection of Authenticated Cell Cultures (ECACC), an international repository managed by the United Kingdom Health Security Agency (UKHSA). No licensing agreement is required when either this cell line or the DNA extracted from it are used for internal research purposes only. Any other use of these products is prohibited without the express written permission of UKHSA. Inquiries regarding authorized use of this cell line or its genomic DNA may be directed to culturecollections@ukhsa.gov.uk.