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About This Item
UNSPSC Code:
41106514
Biological source:
human blood
Relevant disease(s):
cancer
Growth mode:
Suspension
Karyotype:
46XY,+t(8,?13)(p12-21,?q13)+(8,?3)(p12-21,?q21)+I
Morphology:
Lymphoblastoid
biological source
human blood
packaging
tube of 5 μg 96090516-DNA-5UG, pkg of vial of cells 96090516-1VL
growth mode
Suspension
karyotype
46XY,+t(8,?13)(p12-21,?q13)+(8,?3)(p12-21,?q21)+I
morphology
Lymphoblastoid
products
Not specified
receptors
Not specified
technique(s)
cell culture | mammalian: suitable
relevant disease(s)
cancer
shipped in
dry ice
storage temp.
−196°C
Application
Study of B cell leukaemia
Biochem/physiol Actions
Derived from the peripheral blood of a human Caucasian male patient with prolymphocytic leukaemia. Cells have been subsequently transformed by treatment with EBV and TPA (12-0-tetradecanoylphorbol-13-acetate). JVM-13 cells express surface-membrane and cytoplasmic lambda light chain immunoglobulin. Immunological markers detected were HLA-DR, CD19, CD20, CD21, FMC7, CD38, CD71 and CD9.
Human Caucasian B-prolymphocytic leukaemia, EBV transformed
STR-PCR Data: Amelogenin: X,Y
CSF1PO: 10,12
D13S317: 9,11
D16S539: 11
D5S818: 11
D7S820: 9
THO1: 6,7
TPOX: 10,11
vWA: 16,18
CSF1PO: 10,12
D13S317: 9,11
D16S539: 11
D5S818: 11
D7S820: 9
THO1: 6,7
TPOX: 10,11
vWA: 16,18
Preparation Note
RPMI 1640 + 4mM Glutamine + 10% Foetal Bovine Serum (FBS).
Maintain cultures between 3-9x100,000 cells/ml; 5% CO2; 37°C.
Other Notes
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Regulatory Information
常规特殊物品
低风险生物材料
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