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Merck
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JVM-13

NOTE: Both the cell line and DNA from the cell line may be available for this product. Please choose -1VL or VIAL for cells, or -DNA-5UG for DNA.

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关于此项目

UNSPSC Code:
41106514
Biological source:
human blood
Relevant disease(s):
cancer
Growth mode:
Suspension
Karyotype:
46XY,+t(8,?13)(p12-21,?q13)+(8,?3)(p12-21,?q21)+I
Morphology:
Lymphoblastoid
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biological source

human blood

packaging

tube of 5 μg 96090516-DNA-5UG, pkg of vial of cells 96090516-1VL

growth mode

Suspension

karyotype

46XY,+t(8,?13)(p12-21,?q13)+(8,?3)(p12-21,?q21)+I

morphology

Lymphoblastoid

products

Not specified

receptors

Not specified

technique(s)

cell culture | mammalian: suitable

relevant disease(s)

cancer

shipped in

dry ice

storage temp.

−196°C

Application

Study of B cell leukaemia

Biochem/physiol Actions

Derived from the peripheral blood of a human Caucasian male patient with prolymphocytic leukaemia. Cells have been subsequently transformed by treatment with EBV and TPA (12-0-tetradecanoylphorbol-13-acetate). JVM-13 cells express surface-membrane and cytoplasmic lambda light chain immunoglobulin. Immunological markers detected were HLA-DR, CD19, CD20, CD21, FMC7, CD38, CD71 and CD9.
Human Caucasian B-prolymphocytic leukaemia, EBV transformed
STR-PCR Data: Amelogenin: X,Y
CSF1PO: 10,12
D13S317: 9,11
D16S539: 11
D5S818: 11
D7S820: 9
THO1: 6,7
TPOX: 10,11
vWA: 16,18

Preparation Note

RPMI 1640 + 4mM Glutamine + 10% Foetal Bovine Serum (FBS).
Maintain cultures between 3-9x100,000 cells/ml; 5% CO2; 37°C.

Other Notes

Additional freight & handling charges may be applicable for Asia-Pacific shipments. Please check with your local Customer Service representative for more information.

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