biological source
human blood
packaging
tube of 5 μg 96090516-DNA-5UG, pkg of vial of cells 96090516-1VL
growth mode
Suspension
karyotype
46XY,+t(8,?13)(p12-21,?q13)+(8,?3)(p12-21,?q21)+I
morphology
Lymphoblastoid
products
Not specified
receptors
Not specified
technique(s)
cell culture | mammalian: suitable
relevant disease(s)
cancer
shipped in
dry ice
storage temp.
−196°C
Application
Study of B cell leukaemia
Biochem/physiol Actions
Derived from the peripheral blood of a human Caucasian male patient with prolymphocytic leukaemia. Cells have been subsequently transformed by treatment with EBV and TPA (12-0-tetradecanoylphorbol-13-acetate). JVM-13 cells express surface-membrane and cytoplasmic lambda light chain immunoglobulin. Immunological markers detected were HLA-DR, CD19, CD20, CD21, FMC7, CD38, CD71 and CD9.
Human Caucasian B-prolymphocytic leukaemia, EBV transformed
STR-PCR Data: Amelogenin: X,Y
CSF1PO: 10,12
D13S317: 9,11
D16S539: 11
D5S818: 11
D7S820: 9
THO1: 6,7
TPOX: 10,11
vWA: 16,18
CSF1PO: 10,12
D13S317: 9,11
D16S539: 11
D5S818: 11
D7S820: 9
THO1: 6,7
TPOX: 10,11
vWA: 16,18
Preparation Note
Maintain cultures between 3-9x100,000 cells/ml; 5% CO2; 37°C.
RPMI 1640 + 4mM Glutamine + 10% Foetal Bovine Serum (FBS).
Other Notes
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This cell line is part of the European Collection of Authenticated Cell Cultures (ECACC), an international repository managed by the United Kingdom Health Security Agency (UKHSA). No licensing agreement is required when either this cell line or the DNA extracted from it are used for internal research purposes only. Any other use of these products is prohibited without the express written permission of UKHSA. Inquiries regarding authorized use of this cell line or its genomic DNA may be directed to culturecollections@ukhsa.gov.uk.