LB443
NOTE: Both the cell line and DNA from the cell line may be available for this product. Please choose -1VL or VIAL for cells, or -DNA-5UG for DNA.
biological source
human blood
packaging
tube of 5 μg 98062322-DNA-5UG
pkg of vial of cells 98062322-1VL
growth mode
Suspension
karyotype
Not specified
morphology
Lymphoblastoid
products
Not specified
receptors
Not specified
technique(s)
cell culture | mammalian: suitable
shipped in
dry ice
storage temp.
−196°C
Application
Study of DNA repair deficiencies
Biochem/physiol Actions
Established by EBV transformation of mononuclear cell from a peripheral blood sample from a 4 year old male patient. The patient was diagnosed with trichothiodystrophy (TTD), a syndrome connected to deficiency in excision repair of ultraviolet radiation induced DNA damage. Studies with fibroblasts from the same patient, known as TTD4BR, have been published.
Human lymphoblastoid, trichothiodystrophy, EBV-transformed
Preparation Note
Maintain cultures between 3-9 x 100,000 cells/ml, dilute every 1-2 days to 3 x 100,000 cells/ml; 5% CO2, 37°C
RPMI 1640 + 4mM Glutamine + 200 μg/ml Sodium Pyruvate (NaPy) + 15% Heat Inactivated (HI-FBS).
Other Notes
Additional freight & handling charges may be applicable for Asia-Pacific shipments. Please check with your local Customer Service representative for more information.
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