R1

B cell line Rebma HLA A2, Bw60, Cw3,DRw6

Immunofluorescence, Cytometry, Immunoperoxidase, Immunoprecipitation

Mouse x Mouse hybridoma

The hybridoma line R1 has been obtained by fusion of the myeloma P3x63Ag8.653 and spleen cells from (C57Bl/6 x BALB/c)F1 female mice 3 days after a single i.p. injection of lymphoblastoid cells (LCL). The cell line Rebma, which is homozygous for HLA antigens A2, Bw60, Cw3 and DRw6 was used as LCL source. The antibody produced has been analysed in the International Histocompatibility Workshops 10 (1987; 10WS3086)and 11(11WS0936). R1 has been eradicated from mycoplasma using Cibrobay and is also known as R1A1.

Maintain cultures between 3-9 x 100,000 cells/ml; 5% CO₂;37°C

RPMI 1640 + 2mM glutamine + 10% FBS

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This cell line is part of the European Collection of Authenticated Cell Cultures (ECACC), an international repository managed by the United Kingdom Health Security Agency (UKHSA). Genomic DNA is not available for this cell line. Use of this product by commercial organizations may require a license agreement. Commercial organizations will be contacted by cellscreening@merckgroup.com to provide further information after the order request is submitted. Any other use of this cell line is prohibited without the express written permission of UKHSA. Inquiries regarding any other authorized use of this cell line may be directed to culturecollections@ukhsa.gov.uk.