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CHA00011

EUR-2019-nCoV Assays for detection of SARS-CoV-2 RNA

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form

solution (in 10 mM Tris, 1 mM EDTA)

packaging

pkg of 2 vials

pH range

7.5-8.5

shipped in

ambient

storage temp.

−20°C

General description

EUR-2019-nCoV Assays are for qPCR (5′ nuclease assay) detection of SARS-CoV-2.

The product is primers and probes, the sequences of which were designed and made publicly available by Charité Institute of Virology – Universitätsmedizin Berlin.

For additional details, please visit SigmaAldrich.com/ncov.

Features and Benefits

The forward and reverse primers in each tube come with the following features:

  • DNA bases
  • No modifications
  • Desalt purified
  • 8 nmol each E_Sarbeco & 10 nmol each Modified RdRp

The Dual-Labeled Probe in each tube comes with the following features:

  • DNA bases
  • 6-FAM™ reporter
  • BXQ (Block All Quencher—SigmaAldrich.com/bxq)
  • HPLC purified
  • 4 nmol E_Sarbeco & 5 nmol Modified RdRp

Components

The product includes 2 individual tubes of 20X primer and probe pools for quantification of SARS-CoV-2 genes. Assays included are E_Sarbeco and Modified RdRp (formerly Discrimatory) genes.

The forward primer, reverse primer, and probe are at final concentrations of 8, 8, and 4 μM (E_Sarbeco); and, 10, 10, and 5 μM (Modified RdRp), respectively.

E_Sarbeco Assay:
Forward Primer ACAGGTACGTTAATAGTTAATAGCGT
Reverse Primer ATATTGCAGCAGTACGCACACA
Dual-Labeled Probe ACACTAGCCATCCTTACTGCGCTTCG

Modified RdRp Assay:
Forward Primer AAATGGTCATGTGTGGCGGT
Reverse Primer GTTAAAAACACTATTAGCATAAGCAGTTGT
Dual-Labeled Probe CAGGTGGAACCTCATCAGGAGATGC

Legal Information

FAM is a trademark of Applera Corporation or its subsidiaries in the US and/or certain other countries

Storage Class Code

12 - Non Combustible Liquids

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable

Regulatory Information

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