CHROP
Imprint® Ultra Chromatin Optimization Kit
Kit designed to optimize sonication parameters for ChIP experiments
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NACRES:
NA.54
UNSPSC Code:
41116158
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General description
The Imprint Ultra Chromatin Optimization Kit provides reagents needed to optimize chromatin sonication/shearing conditions before performing ChIP. It is imperative that chromatin is completely sheared into fragments with a peak around 500 base pairs (bp) for ChIP-qPCR assays, and < 500 bp for ChIP-Seq and ChIP-chip, for maximum specificity and resolution, and to ensure low background. For higher resolution applications like genome-wide location analysis by ChIP-chip or ChIP-Seq, sonication of input chromatin to below 500 bp, with a peak around 250 bp, is critical.
Other Notes
Sufficient reagents are provided to perform three sets of chromatin sonication optimization experiments. Each set would include five different conditions for sonication to optimize the parameters. Reagents are provided for a total of 15 reactions, including preparation of cell lysates from 107 cells/reaction, nuclei lysis, sonication, cross-link reversal and DNA purification.
Legal Information
Imprint is a registered trademark of Merck KGaA, Darmstadt, Germany
Kit Components Also Available Separately
Product No.
Description
SDS
- I8896IGEPAL® CA-630, Molecular Biology
- S5150Sodium chloride solution, 5 M in H2O, BioReagent, Molecular Biology, suitable for cell culture
- W4502Water, Nuclease-Free Water, for Molecular Biology
- A84564-(2-Aminoethyl)benzenesulfonyl fluoride hydrochloride, ≥97.0% (HPLC)
- P8340Protease Inhibitor Cocktail, for use with mammalian cell and tissue extracts, DMSO solution
- R4642Ribonuclease A from bovine pancreas, (Solution of 50% glycerol, 10mM Tris-HCL pH 8.0)
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Danger
Hazard Classifications
Acute Tox. 4 Oral - Aquatic Acute 1 - Aquatic Chronic 1 - Eye Dam. 1 - Flam. Liq. 3 - Met. Corr. 1 - Ox. Liq. 1 - Resp. Sens. 1 - Skin Corr. 1B - STOT SE 3
target_organs
Central nervous system
Storage Class
5.1A - Strongly oxidizing hazardous materials
flash_point_f
100.4 °F
flash_point_c
38 °C
Regulatory Information
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Henriette O'Geen et al.
BioTechniques, 41(5), 577-580 (2006-12-05)
A single chromatin immunoprecipitation (ChIP) sample does not provide enough DNA for hybridization to a genomic tiling array. A commonly used technique for amplifying the DNA obtained from ChIP assays is ligation-mediated PCR (LM-PCR). However; using this amplification method, we
Yoon-A Kang et al.
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M H Kuo et al.
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Protocols
Chromatin Immunoprecipitation qPCR for studying gene regulation across conditions.
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