CMC0016
BL21(DE3) Electrocompetent Cells
Escherichia coli, rod shaped
Synonym(s):
BL21 strain
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About This Item
UNSPSC Code:
41106202
NACRES:
NA.85
Product Name
BL21(DE3) Electrocompetent Cells, for protein expression
biological source
Escherichia coli
grade
Molecular Biology
growth mode
adherent or suspension
morphology
rod shaped
technique(s)
microbiological culture: suitable
cell transformation
competent cell type: electrocompetent
transformation efficiency: ≥5 × 109 cfu/μg
shipped in
dry ice
storage temp.
−70°C
General description
The BL21(DE3) Electrocompetent Cells are the first to offer high efficiency cloning and high level protein expression in the same cell.
Cloning efficiencies are increased 25-1,000 fold relative to other preparations of BL21 cells, which is essential for construction of complex expression libraries.
Genotype
F – ompT hsdSB (rB- mB-) gal dcm (DE3)
Cloning efficiencies are increased 25-1,000 fold relative to other preparations of BL21 cells, which is essential for construction of complex expression libraries.
Genotype
F – ompT hsdSB (rB- mB-) gal dcm (DE3)
Features and Benefits
The unprecedented transformation efficiency of the BL21(DE3) Electrocompetent Cells (> 5 × 109 cfu/μg) eliminates the need for plasmid transfer from the cloning strain to the expression strain, saving days of work in a typical cloning and expression experiment
Other Notes
- BL21(DE3) electrocompetent cells
- pUC 19 transformation control DNA
- recovery medium for expression
Storage Class Code
10 - Combustible liquids
Regulatory Information
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Felix Nicolaus et al.
eLife, 10 (2021-02-09)
We follow the cotranslational biosynthesis of three multispanning Escherichia coli inner membrane proteins in vivo using high-resolution force profile analysis. The force profiles show that the nascent chain is subjected to rapidly varying pulling forces during translation and reveal unexpected
Julianne M Troiano et al.
eLife, 10 (2021-01-16)
Under high light, oxygenic photosynthetic organisms avoid photodamage by thermally dissipating absorbed energy, which is called nonphotochemical quenching. In green algae, a chlorophyll and carotenoid-binding protein, light-harvesting complex stress-related (LHCSR3), detects excess energy via a pH drop and serves as
Zev A Ripstein et al.
eLife, 9 (2020-01-10)
The ClpXP degradation machine consists of a hexameric AAA+ unfoldase (ClpX) and a pair of heptameric serine protease rings (ClpP) that unfold, translocate, and subsequently degrade client proteins. ClpXP is an important target for drug development against infectious diseases. Although
M'Lynn E Fisher et al.
eLife, 10 (2021-06-03)
The sarco-plasmic reticulum calcium pump (SERCA) plays a critical role in the contraction-relaxation cycle of muscle. In cardiac muscle, SERCA is regulated by the inhibitor phospholamban. A new regulator, dwarf open reading frame (DWORF), has been reported to displace phospholamban
Brandon R Lowe et al.
eLife, 10 (2021-02-02)
Sequencing of cancer genomes has identified recurrent somatic mutations in histones, termed oncohistones, which are frequently poorly understood. Previously we showed that fission yeast expressing only the H3.3G34R mutant identified in aggressive pediatric glioma had reduced H3K36 trimethylation and acetylation
Protocols
Efficient BL21(DE3) electrocompetent cell transformation protocol ensures optimal settings for high efficiency.
Our team of scientists has experience in all areas of research including Life Science, Material Science, Chemical Synthesis, Chromatography, Analytical and many others.
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