CMC0023
CONTROLLER SIG10 Chemically Competent Cells
for controlled protein expression
biological source
Escherichia coli
grade
for molecular biology
growth mode
adherent or suspension
morphology
rod shaped
technique(s)
microbiological culture: suitable
cell transformation
competent cell type: chemically competent
transformation efficiency: >1 × 109 cfu/μg
shipped in
dry ice
storage temp.
−70°C
Related Categories
General description
They are ideal for cloning and propagation of plasmid clones. They give high yield and high quality plasmid DNA due to the endA1 mutation.
Genotype
mcrA Δ(mrr-hsdRMS-mcrBC) endA1 recA1 Φ80dlacZΔM15 ΔlacX74 araD139 Δ (ara,leu)7697 galU galK rpsL (StrR) nupG λ- tonA Mini-F lacIq1 (GentR)
Features and Benefits
- Reduce leaky expression: The LacI repressor protein binds to the lacO operator to block transcription of downstream genes. In the absence of an induction agent such as lactose sugar or IPTG, target protein expression from the adjacent promoter will be greatly reduced.
- High protein yields: Both 10G and BL21(DE3)-based strains are noted for their ability to express high levels of protein. For expression from a T7 promoter please select the BL21(DE3) strain.
- Terrific efficiency: The simple, reliable heat-shock procedure for chemical transformation gives excellent results from small amounts of plasmid DNA.
Components
- CONTROLLER SIG10 chemically competent cells
- pUC 19 transformation control DNA
- recovery medium for expression
related product
Storage Class Code
10 - Combustible liquids
WGK
WGK 3
Regulatory Information
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