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CMR0001

Sigma-Aldrich

Recovery Medium for Expression

for use with competent cells

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NACRES:
NA.85

grade

for molecular biology

sterility

sterile; autoclaved

shipped in

dry ice

storage temp.

−20°C

General description

This recovery media has and optimized formulation for ensuring stability and high-efficiency transformation of protein expression clones.

Features and Benefits

  • Formulated without lactose to prevent unwanted leaky expression from T7 or other lactose/IPTG-inducible promoters.
  • Enables the highest possible transformation efficiency.
  • Use in the outgrowth stage of competent cell transformation to achieve 10-folder higher transformation efficiency than you would get with LB or SOC media.

Storage Class Code

10 - Combustible liquids

Regulatory Information

动植物来源培养基

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Salvatore Di Girolamo et al.
Microbial cell factories, 19(1), 170-170 (2020-08-29)
Miniaturization of biochemical reaction volumes within artificial microcompartments has been the key driver for directed evolution of several catalysts in the past two decades. Typically, single cells are co-compartmentalized within water-in-oil emulsion droplets with a fluorogenic substrate whose conversion allows

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