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CRISPR13H

CRISPR-Lenti Human HPRT1 Positive Control High Titer Transduction Particles

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About This Item

NACRES:
NA.51
UNSPSC Code:
41106609
Technical Service
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form

liquid

packaging

vial of 200 μL

concentration

5x108  VP/ml (via p24 assay)

application(s)

CRISPR

shipped in

dry ice

storage temp.

−70°C

Quality Level

Application

Functional Genomics/Screening/Target Validation
  • Positive control, HPRT1, CRISPR high titer lentivirus format, for creating gene knockouts/genetic modifications in cell lines.

To see more application data, protocols, vector maps visit sigma.com/crispr.

Features and Benefits

Serves as a positive experimental control for the CRISPR editing workflow using lenti CRISPR DNA. The number of cells with HPRT1 knocked out increases over time with 6−TG selection.

Other Notes

This product is for R&D;use only, not for drug, household, or other uses. Please consult the Safety Data Sheet for information regarding hazards and safe handling practices. Though the lentiviral transduction particles produced are replication incompetent, it is recommended that they be treated as Risk Group Level 2 (RGL−2) organisms in laboratory handling. Follow all published RGL−2 guidelines for laboratory handling and waste decontamination.

Physical form

200 μl virus, minimum p24 titer > 5 x 108 TU/mL

Storage Class

12 - Non Combustible Liquids

wgk

WGK 3

flash_point_f

Not applicable

flash_point_c

Not applicable

Regulatory Information

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Protocols

Learn about CRISPR Cas9, what it is and how it works. CRISPR is a new, affordable genome editing tool enabling access to genome editing for all.

了解CRISPR Cas9、定义和工作原理。CRISPR是一种全新、实惠的基因组编辑工具,可让所有人掌握基因组编辑。

Our team of scientists has experience in all areas of research including Life Science, Material Science, Chemical Synthesis, Chromatography, Analytical and many others.

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