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About This Item
NACRES:
NA.51
UNSPSC Code:
41106609
form
liquid
packaging
vial of 200 μL
concentration
5x108 VP/ml (via p24 assay)
application(s)
CRISPR
shipped in
dry ice
storage temp.
−70°C
Quality Level
Related Categories
Application
Functional Genomics/Screening/Target Validation
To see more application data, protocols, vector maps visit sigma.com/crispr.
- Positive control, HPRT1, CRISPR high titer lentivirus format, for creating gene knockouts/genetic modifications in cell lines.
To see more application data, protocols, vector maps visit sigma.com/crispr.
Features and Benefits
Serves as a positive experimental control for the CRISPR editing workflow using lenti CRISPR DNA. The number of cells with HPRT1 knocked out increases over time with 6−TG selection.
Legal Information
Other Notes
This product is for R&D;use only, not for drug, household, or other uses. Please consult the Safety Data Sheet for information regarding hazards and safe handling practices. Though the lentiviral transduction particles produced are replication incompetent, it is recommended that they be treated as Risk Group Level 2 (RGL−2) organisms in laboratory handling. Follow all published RGL−2 guidelines for laboratory handling and waste decontamination.
Physical form
200 μl virus, minimum p24 titer > 5 x 108 TU/mL
Storage Class
12 - Non Combustible Liquids
wgk
WGK 3
flash_point_f
Not applicable
flash_point_c
Not applicable
Regulatory Information
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Protocols
Learn about CRISPR Cas9, what it is and how it works. CRISPR is a new, affordable genome editing tool enabling access to genome editing for all.
了解CRISPR Cas9、定义和工作原理。CRISPR是一种全新、实惠的基因组编辑工具,可让所有人掌握基因组编辑。
Our team of scientists has experience in all areas of research including Life Science, Material Science, Chemical Synthesis, Chromatography, Analytical and many others.
Contact Technical Service