CRISPR18
Lenti CRISPR Universal Non-Target Control#1 Plasmid DNA (LV04 vector)
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About This Item
NACRES:
NA.51
UNSPSC Code:
41106609
form
liquid
packaging
vial of 50 μL
concentration
20 ng/μL in TE buffer; DNA (1μg of plasmid DNA)
application(s)
CRISPR
shipped in
dry ice
storage temp.
−20°C
Quality Level
Related Categories
Legal Information
Other Notes
Ampicillin and puromycin antibiotic resistance genes provide selection in bacterial or mammalian cells, respectively. Self-inactivating replication incompetent viral particles can be produced in packaging cells (HEK293T) by co-transfection with compatible packaging plasmids (Product No. SHP002).
Application
Functional Genomics/Screening/Target Validation
General description
The Lenti CRISPR Non-Target Control#1 (LV04 vector) is a lentiviral plasmid vector, which includes a gRNA sequence that does not target known human, mouse and rat genes. The DNA format of this control construct permits direct transfection in knockout experiments or use in the viral packaging process (for lentiviral-based knockdown experiments). This vector is useful as a negative control in experiments using CRISPR lentiviral clones. The lenti CRISPR non-target control uses a dual-component system consisting of U6-driven guide RNA (non-targeting) and hPGK-driven Puromycin resistance cassette and Blue Fluorescence Protein for fluorophorescence. This control requires Cas9 be delivered by a separate vector.
Storage Class
12 - Non Combustible Liquids
wgk
WGK 2
flash_point_f
Not applicable
flash_point_c
Not applicable
Regulatory Information
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Articles
Genome-wide screening with optimized gRNAs per gene ensures specific and efficient knockout, controlling time and cost.
全基因组功能缺失筛查是发现生物过程背后的基因和途径的有效方法。现在，每个基因都可通过两个优化的 gRNA 完全敲除。通过最大限度减少克隆数量，可确保尽可能特异性的筛选，同时控制时间和成本。
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