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Merck
CN

CS0013

Red Protease Detection Kit

Fluorescent protease assay, high sensitivity

Synonym(s):

Fluorescent Protease Assay Kit, Protease Assay Kit

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About This Item

NACRES:
NA.51
UNSPSC Code:
12350000
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Quality Segment

packaging

pkg of 1 kit

shipped in

dry ice

storage temp.

−20°C

General description

The Red Protease Detection Kit uses TAMRA-casein as a convenient fluorescent substrate for detection of proteases in various samples.

Fluorescence excitation wavelength = 535 nm

Fluorescence emission wavelength = 595 nm

Application

Detection and quantitation of protease activity in samples. Useful for impurity testing.
Detection limit approximately 19 ng/ml, when using a 1 hour incubation with Trypsin as a standard.

Features and Benefits

  • Fluorescence is pH-independent
  • No separation or precipation steps are required
  • High sensitivity and signal-to-noise

Other Notes

CS0013A Trypsin (1 mg/mL): 100 μL

CS0013B Protease AssayBuffer × 2: 30 mL

CS0013C TAMRA-Casein substrate: 280 μL


Storage Class

12 - Non Combustible Liquids

wgk

WGK 1

flash_point_f

Not applicable

flash_point_c

Not applicable



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