General description
The Red Protease Detection Kit uses TAMRA-casein as a convenient fluorescent substrate for detection of proteases in various samples.
Fluorescence excitation wavelength = 535 nm
Fluorescence emission wavelength = 595 nm
Fluorescence excitation wavelength = 535 nm
Fluorescence emission wavelength = 595 nm
Application
Detection and quantitation of protease activity in samples. Useful for impurity testing.
Detection limit approximately 19 ng/ml, when using a 1 hour incubation with Trypsin as a standard.
Features and Benefits
- Fluorescence is pH-independent
- No separation or precipation steps are required
- High sensitivity and signal-to-noise
Other Notes
CS0013A Trypsin (1 mg/mL): 100 μL
CS0013B Protease AssayBuffer × 2: 30 mL
CS0013C TAMRA-Casein substrate: 280 μL
CS0013B Protease AssayBuffer × 2: 30 mL
CS0013C TAMRA-Casein substrate: 280 μL
Storage Class Code
12 - Non Combustible Liquids
WGK
WGK 1
Flash Point(F)
Not applicable
Flash Point(C)
Not applicable
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