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Safety Information

CS0740

Sigma-Aldrich

Acid phosphatase Assay Kit

1 kit sufficient for 1,000 assays (multiwell plates), 1 kit sufficient for 100 assays (tubes)

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eCl@ss:
42010102
NACRES:
NA.84

Quality Level

200

usage

 kit sufficient for 1,000 assays (multiwell plates)
 kit sufficient for 100 assays (tubes)

shipped in

dry ice

storage temp.

−20°C

Gene Information

human ... ACP1(52) , ACP2(53)

Related Categories

Application

The kit is used for the detection of acid phosphatase activity in tissues, whole cell extracts, column fractions and purified enzyme. The kit contains all the reagents required for a fast and simple acid phosphatase detection. The kit contains a standard solution and a control enzyme.

Biochem/physiol Actions

Acid phosphatase is one of the acid hydrolases that normally reside in lysosomes. It is a marker for the identification of lysosomes in sub-cellular fractionations.

Other Notes

2024 CiteAb Award Winner for Supplier Succeeding in Parkinson′s Research

Kit Components Only

Product No.
Description
  • p-Nitrophenyl phosphate tablets 5 mg 20 tablets
  • Citrate buffer solution, 0.09 M pH 4.8 100 mL
  • p-Nitrophenol Standard solution 10 mM 1 mL
  • Acid phosphatase control enzyme .2 mL

Pictograms

Health hazard
GHS08

Signal Word

Warning

Hazard Statements

H351 - H373

Hazard Classifications

Carc. 2 - STOT RE 2 Oral

Storage Class Code

10 - Combustible liquids

WGK

WGK 3

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable

Regulatory Information

含少量动物源组分生物产品
易制毒化学品（2类）

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Xiaogang Xu et al.
Frontiers in immunology, 9, 2321-2321 (2018-11-22)
Glycyrrhizin (GL), a triterpenoid glycoside, serves important functions in various biological activities, including antiviral and antitumor immune responses. However, the anti-inflammatory effects of GL on Salmonella enterica serovar Typhimurium (ST)-induced injury in mice and the mechanisms underlying the protection of
Qinghe Song et al.
PloS one, 10(3), e0120790-e0120790 (2015-03-27)
The Yes-associated protein (YAP), a transcriptional coactivator inactivated by the Hippo tumor suppressor pathway, functions as an oncoprotein in a variety of cancers. However, its contribution to breast cancer remains controversial. This study investigated the role of YAP in breast
Archana Vaithilingam et al.
BMC cell biology, 14, 35-35 (2013-08-14)
Endolysosomes play a key role in maintaining the homeostasis of the cell. They are made of a complex set of proteins that degrade lipids, proteins and sugars. Studies involving endolysosome contribution to cellular functions such as MHC class I and
Jeffrey E McLean et al.
Journal of virology, 83(16), 8233-8246 (2009-06-06)
The ectopic overexpression of Bcl-2 restricts both influenza A virus-induced apoptosis and influenza A virus replication in MDCK cells, thus suggesting a role for Bcl-2 family members during infection. Here we report that influenza A virus cannot establish an apoptotic
Fan Zhang et al.
American journal of physiology. Cell physiology, 301(2), C421-C430 (2011-05-27)
It is well known that the mutation of TRP-ML1 (transient receptor potential-mucolipin-1) causes mucolipidosis IV, a lysosomal storage disease. Given that lysosomal nicotinic acid adenine dinucleotide phosphate (NAADP)-Ca(2+) release channel activity is associated with TRP-ML1, the present study was designed
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Articles

Organelle Isolation

The isolation of subcellular fractions by centrifugation is a commonly used technique and is widely applicable across multiple cell and tissue types. Because organelles differ in their size, shape, and density, centrifugation can be easily employed to separate and purify organelle fractions from gently homogenized samples.

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