CYTOCOX1
Cytochrome c Oxidase Assay Kit
sufficient for 100 tests, soluble and membrane bound mitochondria
Synonym(s):
Cytochrome c Oxidase Activity Assay Kit
Sign In to View Organizational & Contract Pricing.
Select a Size
About This Item
NACRES:
NA.84
UNSPSC Code:
12161503
Usage:
sufficient for 100 tests
Storage temp.:
−20°C
Shipped in:
dry ice
usage
sufficient for 100 tests
shipped in
dry ice
storage temp.
−20°C
Quality Level
Gene Information
human ... COX1(4512)
Related Categories
Application
The Cytochrome c Oxidase Assay Kit uses an optimized colorimetric assay based on observation of the decrease in absorbance of ferrocytochrome c measured at 550 nm, which is caused by its oxidation to ferricytochrome c by cytochrome c oxidase. This kit is suitable for the detection of mitochondrial outer membrane integrity/mitochondrial stress and for the detection of mitochondria in subcellular fractions.
Biochem/physiol Actions
Cytochrome c oxidase [EC 1.9.3.1] is located on the inner mitochondrial membrane dividing the mitochondrial matrix from the intermembrane space, and has traditionally been used as a marker for this membrane. It is also located in the cytoplasmic membrane of bacteria. Cytochrome c oxidase provides energy for the cell by coupling electron transport through the cytochrome chain with the process of oxidative phosphorylation.
Features and Benefits
- Simple, optimized protocol - Obtain reproducible results without special training needs
- Useful for determining cytochrome c activity from any mitchondrial source - The enzyme is present in all mitochondria regardless of species
- Useful for detecting the presence of mitochondria in subcellular fraction - Save time and increase confidence in the quality of organelle preparations
- May be used in conjunction with the MITOISO1 Mitochondrial Isolation Kit - Standardized mitochondrial preparation and analysis ensures reproducibility
- Use to analyze the intactness of mitochondrial membranes
- Simple colorimetric measurement of solution
signalword
Danger
hcodes
Hazard Classifications
Eye Dam. 1 - Skin Irrit. 2
Storage Class
10 - Combustible liquids
Regulatory Information
动植物源性产品
低风险生物材料
This item has
Choose from one of the most recent versions:
Already Own This Product?
Find documentation for the products that you have recently purchased in the Document Library.
Chunpeng Xu et al.
Frontiers in behavioral neuroscience, 12, 238-238 (2018-10-26)
The habenula is a nuclear complex composed of the lateral habenula (LHb) and medial habenula (MHb), two distinct structures. Much progress has been made to emphasize the role of the LHb in the pathogenesis of depression. In contrast, relatively less
Chao-Chin Liu et al.
Frontiers in microbiology, 11, 170-170 (2020-03-03)
Staphylococcus aureus spreads rapidly on the surface of soft agar medium. The spreading depends on the synthesis of biosurfactants, i.e., phenol soluble modulins (PSMs), which facilitate colony spreading of S. aureus. Our earlier study demonstrated that water accumulates in a
Hikaru Miyagi et al.
The Journal of biological chemistry, 284(12), 7553-7560 (2009-01-23)
Cells of the yeast Saccharomyces cerevisiae contain three NAD kinases; namely, cytosolic Utr1p, cytosolic Yef1p, and mitochondrial Pos5p. Previously, the NADH kinase reaction catalyzed by Pos5p, rather than the NAD kinase reaction followed by the NADP(+)-dependent dehydrogenase reaction, had been
Filip J Larsen et al.
FASEB journal : official publication of the Federation of American Societies for Experimental Biology, 25(8), 2843-2852 (2011-05-18)
The basal metabolic rate (BMR) is referred to as the minimal rate of metabolism required to support basic body functions. It is well known that individual BMR varies greatly, even when correcting for body weight, fat content, and thyroid hormone
Céline Aguer et al.
PloS one, 6(12), e28536-e28536 (2011-12-24)
Human primary myotubes are highly glycolytic when cultured in high glucose medium rendering it difficult to study mitochondrial dysfunction. Galactose is known to enhance mitochondrial metabolism and could be an excellent model to study mitochondrial dysfunction in human primary myotubes.
Articles
Centrifugation separates organelles based on size, shape, and density, facilitating subcellular fractionation across various samples.
Cellular apoptosis assays to detect programmed cell death using Annexin V, Caspase and TUNEL DNA fragmentation assays.
细胞凋亡测定使用膜联蛋白V、Caspase和TUNEL DNA片段化检测法检测程序性细胞死亡。
亚细胞组分离心分离是一种常用技术，广泛适用于多种类型的细胞和组织。由于不同细胞器的大小、形状和密度不同，因此通过离心，可从温和均质的样品中轻松分离和纯化细胞器组分。
Related Content
Our team of scientists has experience in all areas of research including Life Science, Material Science, Chemical Synthesis, Chromatography, Analytical and many others.
Contact Technical Service