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D0426

DAB with Metal Enhancer

SIGMAFAST™, peroxidase substrate, tablet

Synonym(s):

3,3′-Diaminobenzidine Peroxidase Substrate, DAB Peroxidase Substrate

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About This Item

UNSPSC Code:
12352204
NACRES:
NA.83
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Product Name

SIGMAFAST™ DAB with Metal Enhancer, tablet

form

tablet

Quality Level

200

solubility

water: 1 tablet/5 mL

storage temp.

−20°C

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General description

SIGMAFAST™ DAB with Metal Enhancer Tablets require no additional buffers or steps to prepare an active substrate solution for detection of peroxidase activity. Commom use is in immunohistology as a precipitating substrate.

Application

SIGMAFAST™ DAB with Metal Enhancer has been used in the visualization of lung tissue sections using immunohistochemistry, dorsal root ganglion (DRG) neurons and immunohistochemical staining for collagen in anterior cruciate ligament (ACL) cells.
The DAB reaction is enhanced by the addition of CoCl2 and produces a more intense stain which is resistant to alcohol.

Preparation Note

Each tablet set dissolved in 5 mL deionized water yields a ready-to-use buffered solution containing DAB with a metal enhancer.

Other Notes

2024 CiteAb Award Winner for Supplier Succeeding in Parkinson′s Research

Legal Information

SIGMAFAST is a trademark of Sigma-Aldrich Co. LLC

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Danger

Hazard Classifications

Aquatic Chronic 2 - Carc. 1B - Eye Dam. 1 - Muta. 2 - Repr. 1B - Resp. Sens. 1 - Skin Sens. 1

Storage Class

6.1C - Combustible acute toxic Cat.3 / toxic compounds or compounds which causing chronic effects

flash_point_f

Not applicable

flash_point_c

Not applicable

wgk

WGK 3

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Certificates of Analysis (COA)

Lot/Batch Number
SLCD4801
SLBP7387V
SLBJ7196V
SLBF4765V
061M8207V

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Platelets and plasma proteins are both required to stimulate collagen gene expression by anterior cruciate ligament cells in three-dimensional culture
Cheng M, et al.
Tissue Engineering: Part A, 16(5), 1479-1489 (2010)
Cdk5 inhibitor roscovitine alleviates neuropathic pain in the dorsal root ganglia by downregulating N-methyl-D-aspartate receptor subunit 2A
Yang L, et al.
Neurological Sciences, 35(9), 1365-1371 (2014)
Somanshu Banerjee et al.
Journal of photochemistry and photobiology. B, Biology, 176, 124-135 (2017-10-11)
In most of the avian species, daylength or photoperiod is the main environmental factor regulating reproduction. During their annual gonadal cycle, birds once sensitive to short or long day effect develop refractoriness to the same daylength and gonad develop or
Developing rat lung has a sided pacemaker region for morphogenesis-related airway peristalsis
Jesudason EC, et al.
American Journal of Respiratory Cell and Molecular Biology, 32(2), 118-127 (2005)
Germain J P Fernando et al.
Journal of controlled release : official journal of the Controlled Release Society, 237, 35-41 (2016-07-07)
DNA vaccines have many advantages such as thermostability and the ease and rapidity of manufacture; for example, in an influenza pandemic situation where rapid production of vaccine is essential. However, immunogenicity of DNA vaccines was shown to be poor in
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Articles

Colorimetric Phosphatase/Peroxidase Substrate Systems

NBT-BCIP substrate system aids in western blotting and immunohistological staining, producing a blue-purple insoluble end product.

比色碱性磷酸酶和过氧化物酶底物检测系统

硝基蓝四唑（NBT）可与碱性磷酸酶底物5-溴-4-氯-3-吲哚基磷酸盐（BCIP）一起用于蛋白印迹和免疫组织染色程序。这些底物系统产生不溶的NBT二甲终产物，其颜色为蓝至紫色，可以通过视觉观察到。

Protocols

Immunohistochemistry Procedure

Use this protocol to for the entire immunohistochemistry (IHC) procedure through staining and visualization of specific antigens in paraffin-embedded tissue sections.

免疫组织化学操作步骤

本实验方案通过对石蜡包埋的组织切片中的特定抗原进行染色和成像，完成整个免疫组织化学（IHC）操作步骤。

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Global Trade Item Number

SKUGTIN
D0426-5SET04061835556427
D0426-50SET04061835506187

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