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D0547

Dulbecco′s Modified Eagle′s Medium/Nutrient Mixture F-12 Ham

With L-glutamine and trace elements, without HEPES and sodium bicarbonate, powder, suitable for cell culture

Synonym(s):

DME/F-12, 1:1 mixture

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About This Item

UNSPSC Code:
12352207
NACRES:
NA.71
MDL number:
MFCD00217417

Key Documents

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Product Name

Dulbecco′s Modified Eagle′s Medium/Nutrient Mixture F-12 Ham, With L-glutamine and trace elements, without HEPES and sodium bicarbonate, powder, suitable for cell culture

form

powder

technique(s)

cell culture | mammalian: suitable

components

NaHCO3: no
sodium pyruvate: 0.11 g/L
HEPES: no
phenol red: yes
glucose: 3.15 g/L
L-glutamine: yes

shipped in

ambient

storage temp.

2-8°C

Quality Level

500

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Application

Dulbecco′s Modified Eagle′s Medium/Nutrient Mixture F-12 Ham has been used:

  • to culture cerebral cortices from mice embryos
  • as a component of the human corneal epithelium (HCE) media for culturing human limbal explants
  • to culture human corneal epithelial cell lines

General description

Dulbecco′s Modified Eagle′s Medium/Nutrient Mixture F-12 Ham is a 1:1 mixture.

Other Notes

Trace elements include ammonium molybdate, ammonium metavanadate, stannous chloride, sodium selenite, magnesium sulfate, nickel chloride, and sodium metasilicate

Preparation Note

Formulated to contain 12.0 grams of powder per liter of medium.
Supplement with 1.2 g/L sodium bicarbonate

Regulatory Information

动植物来源培养基
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Certificates of Analysis (COA)

Lot/Batch Number
0000456195
0000456195
0000456192
0000456192
25C103

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Optimizing the role of limbal explant size and source in determining the outcomes of limbal transplantation: An in vitro study
Abhinav Reddy K, et al.
PLoS ONE (2017)
LPA2 promotes neuronal differentiation and neurite formation in neocortical development
Mizuki U, et al.
Biochemical and Biophysical Research Communications, 89-94 (2022)
Christo J Botha et al.
The Onderstepoort journal of veterinary research, 87(1), e1-e4 (2020-04-04)
Diplodiosis is an important neuromycotoxicosis of ruminants in South Africa when grazing on harvested maize fields in winter. It is believed to be caused by mycotoxin(s) synthesised by Stenocarpella (Diplodia) maydis. Although several metabolites have been isolated from S. maydis
Ceyda Acilan et al.
Biochimica et biophysica acta, 1861(2), 218-234 (2016-10-25)
To overcome the hurdles of cisplatin, majorly its toxicity and resistance, there has been extensive search for alternative anti-cancer metal-based compounds. Here, three Cu(II)-complexes, Cu(Sal-Gly)(phen), Cu(Sal-Gly)(pheamine), Cu(Sal-Gly)(phepoxy) are characterized for their interaction with DNA, cytotoxicity and mechanism of action. The
Selma M Soyal et al.
Molecular neurobiology, 57(2), 752-764 (2019-09-01)
PPARGC1A encodes a transcriptional co-activator also termed peroxisome proliferator-activated receptor (PPAR) gamma coactivator 1-alpha (PGC-1α) which orchestrates multiple transcriptional programs. We have recently identified CNS-specific transcripts that are initiated far upstream of the reference gene (RG) promoter. The regulation of
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Articles

Nutrient Mixture F-12 Ham Formulation

Nutrient Mixture F-12 Ham Formulation

Protocols

Powdered Media Preparation Instructions

Powdered media and salt mixtures are extremely hygroscopic and should be protected from atmospheric moisture.

粉末培养基制备说明

粉末培养基及盐混合物极具吸湿性，应注意防潮。

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