Merck
CN
All Photos(1)

Documents

Safety Information

D0547

Sigma-Aldrich

Dulbecco′s Modified Eagle′s Medium/Nutrient Mixture F-12 Ham

With L-glutamine and trace elements, without HEPES and sodium bicarbonate, powder, suitable for cell culture

Sign Into View Organizational & Contract Pricing
All Photos(1)
Synonym(s):
DME/F-12, 1:1 mixture
MDL number:
MFCD00217417

Quality Level

500

form

powder

technique(s)

cell culture | mammalian: suitable

components

NaHCO3: no
sodium pyruvate: 0.11 g/L
HEPES: no
phenol red: yes
glucose: 3.15 g/L
L-glutamine: yes

shipped in

ambient

storage temp.

2-8°C

Looking for similar products? Visit Product Comparison Guide

Related Categories

General description

Dulbecco′s Modified Eagle′s Medium/Nutrient Mixture F-12 Ham is a 1:1 mixture.

Application

Dulbecco′s Modified Eagle′s Medium/Nutrient Mixture F-12 Ham has been used:

  • to culture cerebral cortices from mice embryos
  • as a component of the human corneal epithelium (HCE) media for culturing human limbal explants
  • to culture human corneal epithelial cell lines

Other Notes

Trace elements include ammonium molybdate, ammonium metavanadate, stannous chloride, sodium selenite, magnesium sulfate, nickel chloride, and sodium metasilicate

Quantity

Formulated to contain 12.0 grams of powder per liter of medium.

Reconstitution

Supplement with 1.2 g/L sodium bicarbonate

also commonly purchased with this product

Product No.
Description
Pricing

supplement

Product No.
Description
Pricing

Regulatory Information

动植物来源培养基

Certificates of Analysis (COA)

Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.

Already Own This Product?

Documents related to the products that you have purchased in the past have been gathered in the Document Library for your convenience.

Visit the Document Library

Difficulty Finding Your Product Or Lot/Batch Number?

Product numbers are combined with Pack Sizes/Quantity when displayed on the website (example: T1503-25G). Please make sure you enter ONLY the product number in the Product Number field (example: T1503).

Example:

T1503
Product Number
-
25G
Pack Size/Quantity

Additional examples:

705578-5MG-PW

PL860-CGA/SHF-1EA

MMYOMAG-74K-13

1000309185

enter as 1.000309185)

Having trouble? Feel free to contact Technical Service for assistance.

Lot and Batch Numbers can be found on a product's label following the words 'Lot' or 'Batch'.

Aldrich Products

  • For a lot number such as TO09019TO, enter it as 09019TO (without the first two letters 'TO').

  • For a lot number with a filling-code such as 05427ES-021, enter it as 05427ES (without the filling-code '-021').

  • For a lot number with a filling-code such as STBB0728K9, enter it as STBB0728 without the filling-code 'K9'.

Not Finding What You Are Looking For?

In some cases, a COA may not be available online. If your search was unable to find the COA you can request one.

Request COA

Optimizing the role of limbal explant size and source in determining the outcomes of limbal transplantation: An in vitro study
Abhinav Reddy K, et al.
PLoS ONE (2017)
LPA2 promotes neuronal differentiation and neurite formation in neocortical development
Mizuki U, et al.
Biochemical and Biophysical Research Communications, 89-94 (2022)
Ceyda Acilan et al.
Biochimica et biophysica acta, 1861(2), 218-234 (2016-10-25)
To overcome the hurdles of cisplatin, majorly its toxicity and resistance, there has been extensive search for alternative anti-cancer metal-based compounds. Here, three Cu(II)-complexes, Cu(Sal-Gly)(phen), Cu(Sal-Gly)(pheamine), Cu(Sal-Gly)(phepoxy) are characterized for their interaction with DNA, cytotoxicity and mechanism of action. The
Christo J Botha et al.
The Onderstepoort journal of veterinary research, 87(1), e1-e4 (2020-04-04)
Diplodiosis is an important neuromycotoxicosis of ruminants in South Africa when grazing on harvested maize fields in winter. It is believed to be caused by mycotoxin(s) synthesised by Stenocarpella (Diplodia) maydis. Although several metabolites have been isolated from S. maydis
Selma M Soyal et al.
Molecular neurobiology, 57(2), 752-764 (2019-09-01)
PPARGC1A encodes a transcriptional co-activator also termed peroxisome proliferator-activated receptor (PPAR) gamma coactivator 1-alpha (PGC-1α) which orchestrates multiple transcriptional programs. We have recently identified CNS-specific transcripts that are initiated far upstream of the reference gene (RG) promoter. The regulation of
View All Related Papers

Articles

Nutrient Mixture F-12 Ham Formulation

Nutrient Mixture F-12 Ham Formulation

Protocols

Powdered Media Preparation Instructions

Powdered media and salt mixtures are extremely hygroscopic and should be protected from atmospheric moisture.

Our team of scientists has experience in all areas of research including Life Science, Material Science, Chemical Synthesis, Chromatography, Analytical and many others.

Contact Technical Service