D1878
2,6-Dichloroindophenol sodium salt hydrate
suitable for vitamin C determination, BioReagent
product line
BioReagent
Quality Level
form
powder
solubility
H2O: soluble 10 mg/mL, clear, blue to very deep blue
suitability
suitable for vitamin C determination
application(s)
diagnostic assay manufacturing
food and beverages
hematology
histology
storage temp.
room temp
SMILES string
O.[Na+].[O-]c1ccc(cc1)\N=C2/C=C(Cl)C(=O)C(Cl)=C2
InChI
1S/C12H7Cl2NO2.Na.H2O/c13-10-5-8(6-11(14)12(10)17)15-7-1-3-9(16)4-2-7;;/h1-6,16H;;1H2/q;+1;/p-1
InChI key
XHSOLXWCGCVQHE-UHFFFAOYSA-M
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General description
Application
- As redox dye to investigate the efficiency of gold nanoparticles (Au-NP) for enzymatic activity of glucose oxidase (GOx).
- To compose UVB specific dosimeter.
- Quantification of vitamin C (L-ascorbic acid) in fruit juices.
- As substrate in NQO1 assay to investigate the liver cytosolic NQO1 activity spectrophotometrically.
- Mitochondrial succinate CoQ oxidoreductase (complex II) activity assay.
Storage Class Code
11 - Combustible Solids
WGK
WGK 3
Flash Point(F)
Not applicable
Flash Point(C)
Not applicable
Personal Protective Equipment
Certificates of Analysis (COA)
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If available for a given product, the recommended re-test date or the expiration date can be found on the Certificate of Analysis.
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What is the solubility for Product D1878, 2,6-Dichloroindophenol sodium salt hydrate?
During quality control testing, Sigma Aldrich tests solubility at 10 mg/mL in water. Conn's Biological Stains, 10th Edition states that dichloroindophenol sodium salt is soluble up to 3% in water and up to 2% in ethanol.
Has Product D1878, 2,6-Dichloroindophenol sodium salt hydrate, been used as a pH or redox indicator?
Yes. Conn's Biological Stains indicates Dichloroindophenol sodium salt in acid is red and blue in alkali. The product is colorless when reduced.
Other than use as a reagent for determination of Vitamin C, are there other reported uses of Product D1878, 2,6-Dichloroindophenol sodium salt hydrate?
Yes. Conn's Biological Stains indicates Dichloroindophenol sodium salt has been used as an electron receptor, for use in demonstrating NADPH diaphorase and to stain glutathione reductase electrophoresis gels.
Are there substances known to interfere in Vitamin C determination?
Yes. Interfering substances are ferrous, stannous, and cuprous sulfite or thiosulfate ions, and SO2.
Are the absorbance maxima and the extinction coefficients available for Product D1878, 2,6-Dichloroindophenol sodium salt hydrate?
Our Analytical Department has run absorbance spectra on many lots of D1878. Using 0.1 M phosphate buffer, pH 8.3 as the solvent, absorbance maxima in the range of 600-605 nm and a mM extinction coefficient of 18.0-19.7 have been reported.
Are there published references for Dichloroindophenol sodium salt being used for the determination of Vitamin C?
Yes. Please see: W. Horowitz ed. Official Methods of Analysis of the AOAC Washington D.C. , 777, (1970) and K. Hiromi et al., Anal. Biochem. 101, 421, (1980).
Has Sigma Aldrich performed solution stability studies for Product D1878, 2,6-Dichloroindophenol sodium salt hydrate?
No. Unfortunately, we have not performed solution stability studies on this product.
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