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D1925

Demecolcine solution

Name: Demecolcine solution
Brand: SIGMA

10 μg/mL in HBSS, ACF Qualified, BioXtra

Synonym(s):

Colcemid^™ solution, N-Deacetyl-N-methylcolchicine solution

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About This Item

CAS Number:

477-30-5

NACRES:

NA.75

PubChem Substance ID:

24893569

UNSPSC Code:

12352207

MDL number:

MFCD00075459

Form:

liquid

Quality level:

400

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Properties

Quality Level

400

sterility

sterile-filtered

product line

BioXtra

form

liquid

concentration

10 μg/mL in HBSS

technique(s)

cell culture | mammalian: suitable

storage temp.

2-8°C

SMILES string

CN[C@H]1CCc2cc(OC)c(OC)c(OC)c2C3=CC=C(OC)C(=O)C=C13

InChI

1S/C21H25NO5/c1-22-15-8-6-12-10-18(25-3)20(26-4)21(27-5)19(12)13-7-9-17(24-2)16(23)11-14(13)15/h7,9-11,15,22H,6,8H2,1-5H3/t15-/m0/s1

InChI key

NNJPGOLRFBJNIW-HNNXBMFYSA-N

Description

Application

Used for cell synchronization by arresting them at metaphase. Qualified for use in animal component free applications.

Legal Information

Colcemid is a trademark of Ciba-Geigy Corporation

Storage Class

12 - Non Combustible Liquids

wgk

WGK 1

flash_point_f

Not applicable

flash_point_c

Not applicable

ppe

Eyeshields, Gloves, multi-purpose combination respirator cartridge (US)

Regulatory Information

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Phenotypes and karyotypes of human malignant mesothelioma cell lines.

Vandana Relan et al.

PloS one, 8(3), e58132-e58132 (2013-03-22)

Malignant mesothelioma is an aggressive tumour of serosal surfaces most commonly pleura. Characterised cell lines represent a valuable tool to study the biology of mesothelioma. The aim of this study was to develop and biologically characterise six malignant mesothelioma cell

Antisense Oligonucleotides Used to Identify Telomeric G-Quadruplexes in Metaphase Chromosomes and Fixed Cells by Fluorescence Lifetime Imaging Microscopy of o-BMVC Foci.

Ting-Yuan Tseng et al.

Molecules (Basel, Switzerland), 25(18) (2020-09-11)

Identification of the existence of G-quadruplex (G4) structure, from a specific G-rich sequence in cells, is critical to the studies of structural biology and drug development. Accumulating evidence supports the existence of G4 structure in vivo. Particularly, time-gated fluorescence lifetime

The PCNA-associated protein PARI negatively regulates homologous recombination via the inhibition of DNA repair synthesis.

Peter Burkovics et al.

Nucleic acids research, 44(7), 3176-3189 (2016-01-23)

Successful and accurate completion of the replication of damage-containing DNA requires mainly recombination and RAD18-dependent DNA damage tolerance pathways. RAD18 governs at least two distinct mechanisms: translesion synthesis (TLS) and template switching (TS)-dependent pathways. Whereas TS is mainly error-free, TLS

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Global Trade Item Number

SKU	GTIN
D1925-20ML	04061833561072
D1925-100ML	04061838259271