D2001
Deoxyribonucleic acid sodium salt from Escherichia coli strain B
Type VIII
Synonym(s):
DNA
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About This Item
CAS Number:
UNSPSC Code:
41106305
eCl@ss:
32160414
EC Number:
297-226-7
NACRES:
NA.51
MDL number:
Product Name
Deoxyribonucleic acid sodium salt from Escherichia coli strain B, Type VIII
type
Type VIII
form
fibers
impurities
<2% protein
Quality Level
color
, white to white with yellow cast
solubility
water: 0.98-1.02 mg/mL, faintly hazy to hazy, colorless to light yellow
λmax
256-264 nm (15mM NaCl+1.5mM Trisodium Citrate PH7)
storage temp.
2-8°C
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Application
Escherichia coli DNA has many uses in research. Escherichia coli DNA (ecDNA) is used in studies of DNA binding agents that modulate DNA structure and function. E. coli DNA is used in physicochemical studies of DNA behavior in solution, and to evaluate species-specific DNA structures and binding domains. This DNA is particularly useful for genomic analysis, including PCR, library construction in bacteriophage lambda, and in heterologous pre-hybridization and hybridization protocols.
Deoxyribonucleic acid sodium salt from Escherichia coli strain B has been used to measure the melting temperature (Tm) and in differential scanning calorimetry (DSC) studies. It has also been used as a template sequence in the polymerase chain reaction (PCR).
Preparation Note
Prepared by procedure of Marmur, J., J. Mol. Biol., 3, 208 (1961).
Storage Class
11 - Combustible Solids
wgk
WGK 3
flash_point_f
Not applicable
flash_point_c
Not applicable
ppe
Eyeshields, Gloves, type N95 (US)
Regulatory Information
常规特殊物品
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R Richins et al.
Biotechnology progress, 17(2), 252-257 (2001-04-21)
The Escherichia coli DNA binding protein FIS is a transcriptional modulator involved in the regulation of many cellular processes, including the activation of rRNA synthesis. High-level overproduction of FIS in early, mid, or late log cultures resulted in growth-phase- and
Corbin J England et al.
Analytical biochemistry, 616, 114099-114099 (2021-01-04)
Precipitation of DNA is performed frequently in molecular biology laboratories for the purpose of purification and concentration of samples and also for transfer of DNA into cells. Metal ions are used to facilitate these processes, though their precise functions are
Geoffrey S Briggs et al.
The Journal of biological chemistry, 282(17), 12353-12357 (2007-02-20)
The DNA-binding protein, RdgC, is associated with recombination and replication fork repair in Escherichia coli and with the virulence-associated, pilin antigenic variation mediated by RecA and other recombination proteins in Neisseria species. We solved the structure of the E. coli
William G Dundon et al.
International journal of medical microbiology : IJMM, 291(6-7), 545-550 (2002-03-14)
Infection of the stomach mucosa by the gastric pathogen Helicobacter pylori is accompanied by a large infiltration of neutrophils and monocytes which are believed to contribute substantially to H. pylori-induced gastritis. A protein was identified (HP-NAP for neutrophil-activating protein from
Differential scanning calorimetry of bacteria
Miles CA, et al.
Microbiology, 132(4), 939-952 (1986)
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