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Sigma-Aldrich

Histodenz

nonionic density gradient medium

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Synonym(s):
5-(N-2,3-Dihydroxypropylacetamido)-2,4,6-triiodo-N,N′-bis(2,3-dihydroxypropyl)isophthalamide
Empirical Formula (Hill Notation):
C19H26I3N3O9
CAS Number:
Molecular Weight:
821.14
Beilstein:
2406632
EC Number:
MDL number:
PubChem Substance ID:
NACRES:
NA.75

Quality Level

form

powder

application(s)

hematology
histology

storage temp.

room temp

SMILES string

CC(=O)N(CC(O)CO)c1c(I)c(C(=O)NCC(O)CO)c(I)c(C(=O)NCC(O)CO)c1I

InChI

1S/C19H26I3N3O9/c1-8(29)25(4-11(32)7-28)17-15(21)12(18(33)23-2-9(30)5-26)14(20)13(16(17)22)19(34)24-3-10(31)6-27/h9-11,26-28,30-32H,2-7H2,1H3,(H,23,33)(H,24,34)

InChI key

NTHXOOBQLCIOLC-UHFFFAOYSA-N

General description

HistoDenz, also known as iohexol, is a non-ionic X-ray contrast compound developed specifically as an iodinated density gradient medium.

Application

HistoDenz is suitable for separating everything from macromolecules to viable cells by either rate-zonal or isopycnic centrifugation.
  • It has been used in the isolation and culturing of hepatic stellate cells.
  • It has also been employed in the extraction of microbial cells from environmental samples.
  • It has been used in LacZ (β-galactosidase) staining.
  • It has also been used for the enrichment of gonocytes.

Features and Benefits

HistoDenz offers several favorable properties as a gradient medium.
  • It has very low toxicity, low osmolality, high density, and low viscosity (compared to sucrose).
  • It is water-soluble, non-ionic, and chemically near-inert.

Legal Information

Histodenz is a trademark of Sigma-Aldrich Co. LLC

Storage Class Code

11 - Combustible Solids

WGK

WGK 1

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable

Personal Protective Equipment

dust mask type N95 (US), Eyeshields, Gloves

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Interleukin?10 promotes primary rat hepatic stellate cell senescence by upregulating the expression levels of p53 and p21
Huang, et al.
Molecular Medicine Reports, 17 (2018)
Attachment of Pseudomonas putida onto differently structured kaolinite minerals: A combined ATR-FTIR and 1H NMR study.
Vasiliadou I A, et al.
Colloids and Surfaces. B, Biointerfaces, 84(2), 354-359 (2011)
Skeletal muscle tissue clearing for lacz and fluorescent reporters, and immunofluorescence staining.
Verma M, et al.
Methods in Molecular Biology, 129-140 (2016)
SnapShot: Tissue Clearing
Douglas S. Richardson, Jeff W. Lichtman
Cell, 171 (2017)
Extraction of Microbial Cells from Environmental Samples for FISH Approaches
Jennifer Pratscher
Methods in Molecular Biology, 2246 (2021)

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