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Merck
CN

D2196

DAF-FM solution

5 mM in DMSO, ≥97% (HPLC)

Synonym(s):

4-Amino-5-methylamino- 2′,7′-difluorofluorescein, Diaminofluorescein-FM

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About This Item

Empirical Formula (Hill Notation):
C21H14F2N2O5
CAS Number:
254109-20-1
Molecular Weight:
412.34
MDL number:
MFCD29073049
UNSPSC Code:
12352200
NACRES:
NA.77

Key Documents

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Product Name

DAF-FM solution, 5 mM in DMSO, ≥97% (HPLC)

SMILES string

Fc1cc2c(cc1O)Oc3c(cc(c(c3)O)F)C42OC(=O)c5c4ccc(c5N)NC

InChI

1S/C21H14F2N2O5/c1-25-13-3-2-8-18(19(13)24)20(28)30-21(8)9-4-11(22)14(26)6-16(9)29-17-7-15(27)12(23)5-10(17)21/h2-7,25-27H,24H2,1H3

InChI key

DIJCILWNOLHJCG-UHFFFAOYSA-N

assay

≥97% (HPLC)

form

DMSO solution

storage condition

desiccated

color

colorless to yellow, including pink

shipped in

wet ice

storage temp.

−20°C

Quality Level

100

Application

DAF-FM solution has been used as a fluorescent probe for detecting nitric oxide (NO) from plant samples.

Biochem/physiol Actions

DAF-FM is a highly sensitive, photo-stable fluorescent probe for the detection of nitric oxide (NO).
DAF-FM is a highly sensitive, photo-stable fluorescent probe for the detection of nitric oxide (NO). DAF-2 is the original compound and is widely used, but DAF-FM is more sensitive to nitric oxide (NO) compared with DAF-2. DAF-FM (Ex: 500 nm; Em: 515 nm; dectection limit: 3 nM) is more photo-stable and less pH sensitive than DAF-2. DAF-FM is not quite as cell-permeable as the diacetate derivative, but it does not need to be activated by cytosolic enzymes.

Features and Benefits

This compound is featured on the Nitric Oxide Synthases page of the Handbook of Receptor Classification and Signal Transduction. To browse other handbook pages, click here.

Storage Class

11 - Combustible Solids

wgk

WGK 3

flash_point_f

Not applicable

flash_point_c

Not applicable

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Certificates of Analysis (COA)

Lot/Batch Number
0000058944
0000014837
0000012834
025M4706V
023M4702V

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Detection of protein S-nitrosothiols (SNOs) in plant samples on diaminofluorescein (DAF) gels
Rodriguez-Ruiz M, et al.
Bio-protocol null
Marta Rodríguez-Ruiz et al.
Bio-protocol, 7(18), e2559-e2559 (2017-09-20)
In plant cells, the analysis of protein S-nitrosothiols (SNOs) under physiological and adverse stress conditions is essential to understand the mechanisms of Nitric oxide (NO)-based signaling. We adapted a previously reported protocol for detecting protein SNOs in animal systems (

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感应染料

一氧化氮 (NO) 是神经元、内皮细胞和免疫系统中的信号转运分子。

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