D2321
DAF-FM DA solution
5 mM in DMSO, ≥97% (HPLC)
Synonym(s):
4-Amino-5-methylamino- 2′,7′-difluorofluorescein diacetate, Diaminofluorescein-FM diacetate
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About This Item
Empirical Formula (Hill Notation):
C25H18F2N2O7
CAS Number:
Molecular Weight:
496.42
MDL number:
UNSPSC Code:
12352200
NACRES:
NA.77
Assay:
≥97% (HPLC)
Form:
DMSO solution
Storage condition:
desiccated, protect from light
Quality Segment
assay
≥97% (HPLC)
form
DMSO solution
storage condition
desiccated, protect from light
color
clear colorless
shipped in
wet ice
storage temp.
−20°C
SMILES string
Fc1cc2c(cc1OC(=O)C)Oc3c(cc(c(c3)OC(=O)C)F)C42OC(=O)c5c4ccc(c5N)NC
InChI
1S/C25H18F2N2O7/c1-10(30)33-20-8-18-13(6-15(20)26)25(12-4-5-17(29-3)23(28)22(12)24(32)36-25)14-7-16(27)21(34-11(2)31)9-19(14)35-18/h4-9,29H,28H2,1-3H3
InChI key
BEVHTVRRVVEMEF-UHFFFAOYSA-N
Biochem/physiol Actions
DAF-FM DA is a cell permeable fluorescent probe for the detection of nitric oxide (NO).
DAF-FM DA is a cell-permeable fluorescent probe for the detection of nitric oxide (NO). DAF-2 is the original compound and is widely used, but DAF-FM and DAF-FM DA are more sensitive to nitric oxide (NO) compared with DAF-2 and more photo-stable and less pH sensitive. DAF-FM DA permeates well into living cells and is rapidly transformed into water-soluble DAF-FM by cytosolic esterases.
Features and Benefits
This compound is featured on the Nitric Oxide Synthases page of the Handbook of Receptor Classification and Signal Transduction. To browse other handbook pages, click here.
Other Notes
Light sensitive
Storage Class
10 - Combustible liquids
wgk
WGK 3
flash_point_f
Not applicable
flash_point_c
Not applicable
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