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Merck
CN

D2653

Dimethyl sulfoxide

Hybri-Max™, 0.2 μm filtered, BioReagent, suitable for hybridoma, ≥99.7%, Ampoule

Synonym(s):

Dimethyl sulfoxide, DMSO

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About This Item

Linear Formula:
(CH3)2SO
CAS Number:
67-68-5
Molecular Weight:
78.13
UNSPSC Code:
12191502
Beilstein/REAXYS Number:
506008
MDL number:
MFCD00002089
NACRES:
NA.21
Assay:
≥99.7%
Grade:
Hybri-Max™
Form:
liquid
Solubility:
H2O: miscible (completely)

Key Documents

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InChI key

IAZDPXIOMUYVGZ-UHFFFAOYSA-N

SMILES string

CS(C)=O

InChI

1S/C2H6OS/c1-4(2)3/h1-2H3

grade

Hybri-Max™

vapor density

2.7 (vs air)

vapor pressure

0.42 mmHg ( 20 °C)

sterility

0.2 μm filtered

product line

BioReagent

assay

≥99.7%

form

liquid

autoignition temp.

573 °F

expl. lim.

42 %, 63 °F

technique(s)

cell culture | hybridoma: suitable, cryopreservation: suitable

impurities

endotoxin, tested, ≤0.1% water

refractive index

n20/D 1.479 (lit.)

bp

189 °C (lit.)

mp

16-19 °C (lit.)

solubility

H2O: miscible (completely)

density

1.10 g/mL (lit.)

Quality Level

300

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Application

DMSO is a polar aprotic solvent used in chemical reactions, in polymerase chain reactions (PCR) and as a cryoprotectant vitrification agent for the preservation of cells, tissues and organs. DMSO is used in cell freezing media to protect cells from ice crystal induced mechanical injury. It is used for frozen storage of primary, sub-cultured, and recombinant heteroploid and hybridoma cell lines; embryonic stem cells (ESC), and hematopoietic stem cells. DMSO is frequently used in the combinations with BSA or fetal bovine serum (FBS).

Disclaimer

Supercools easily and remelts slowly at room temperature. Solidified product can be re-liquified by warming to room temperature without detriment to the product.

Other Notes

This product is a Hybri-Max product. It is hybridoma tested and is assessed for suitability in cell freezing. This product is 0.2 micron filtered and tested for endotoxin levels.

Packaging

5 mL and 10 mL in flame sealed ampules

Legal Information

Hybri-Max is a trademark of Sigma-Aldrich Co. LLC

Storage Class

10 - Combustible liquids

flash_point_f

188.6 °F - closed cup

flash_point_c

87 °C - closed cup

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