D2812
REDTaq® Genomic DNA Polymerase
without MgCl2
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About This Item
Concentration:
1 unit/μL
Quality Level
form
liquid
usage
sufficient for 1000 reactions, sufficient for 250 reactions, sufficient for 2500 reactions
feature
Difficult Templates/Specialty Enzymes PCR, dNTPs included: no, hotstart: no, Standard PCR
concentration
1 unit/μL
technique(s)
PCR: suitable
color
red
input
purified DNA
application(s)
agriculture
shipped in
wet ice
storage temp.
−20°C
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General description
REDTaq® Genomic DNA Polymerase is a unique blend of Taq DNA Polymerase with an inert red dye. This special formulation is designed to provide enhanced amplification of more complex or genomic templates. REDTaq Genomic DNA Polymerase is highly sensitive, produces increased yields and is capable of generating longer product lengths. It has all the advantages of REDTaq DNA polymerase, such as easy visualization of enzyme addition and complete reaction mixing, and direct loading to an agarose gel. The inert red dye does not effect automated or manual sequencing, restriction digestions or other downstream applications. The dye can easily removed by any standard purification method.
Application
REDTaq® Genomic DNA Polymerase has been used as a component of the polymerase chain reaction (PCR) mix for PCR amplification. It has also been used as a component of the preamplification mix for PCR in amplified fragment length polymorphism (AFLP) analysis of Fusarium oxysporum sp. isolates.
Features and Benefits
- Enhanced amplification on genomic and difficult DNA templates
- Same great performance as Taq DNA Polymerase in a more convenient format for high throughput applications
- Quick recognition and confirmation of appropriate mixing
- No loading buffers or tracking dyes necessary. Sample can be taken directly from reaction and loaded onto an agarose gel
- The samples can be re-amplified as in nested PCR
- The red dye migrates faster than bromophenol blue
- Greater consistency across reactions due to proper mixing
Other Notes
Includes 10× PCR reaction buffer without MgCl2 and a separate tube of 25 mM MgCl2
One unit incorporates 10 nmol of total dNTPs into acid precipitable DNA in 30 min. at 74°C.
Legal Information
Use of this product is covered by one or more of the following US patents and corresponding patent claims outside the US: US 8,404,464 and US 7,972,828. The purchase of this product includes a limited, non-transferable immunity from suit under the foregoing patent claims.
REDTaq is a registered trademark of Merck KGaA, Darmstadt, Germany
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Danger
hcodes
pcodes
Hazard Classifications
Resp. Sens. 1
Storage Class
10 - Combustible liquids
wgk
WGK 1
Regulatory Information
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Paola Persico et al.
Veterinary dermatology, 22(6), 521-527 (2011-06-10)
Ulcerative dermatitis caused by feline herpes virus 1 (FHV-1) is an uncommon disease characterized by cutaneous ulcers secondary to epidermal, adnexal and dermal necrosis. Differential diagnoses for FHV-1 lesions include, but are not limited to, mosquito bite hypersensitivity and eosinophilic
Characterization of Fusarium oxysporum f. sp. radicis-cucumerinum attacking melon under natural conditions in Greece
Vakalounakis DJ
Plant Pathology, 54(3), 339-346 (2005)
Wiesław Bogdanowicz et al.
Proceedings of the National Academy of Sciences of the United States of America, 106(30), 12279-12282 (2009-07-09)
We report the results of mitochondrial and nuclear DNA analyses of skeletal remains exhumed in 2005 at Frombork Cathedral in Poland, that are thought to be those of Nicolaus Copernicus (1473-1543). The analyzed bone remains were found close to the
K L Mealey et al.
Journal of veterinary pharmacology and therapeutics, 38(5), 429-433 (2015-02-11)
The aim of this study was to sequence all exons of the ABCB1 (MDR1) gene in cats that had experienced adverse reactions to P-glycoprotein substrate drugs (phenotyped cats). Eight phenotyped cats were included in the study consisting of eight cats
Protocols
Reactions using REDTaq® DNA polymerase are formulated as any PCR mixtures. There are no additional reaction preparation steps or protocol changes required.
Global Trade Item Number
| SKU | GTIN |
|---|---|
| D2812-1KU | 04061833562604 |
| D2812-250UN | 04061833562628 |
| D2812-2.5KU | 04061833562611 |
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