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D2906

DMEM/F-12

Name: DMEM/F-12
Brand: SIGMA

With ʟ-glutamine, sodium pyruvate and HEPES, without sodium bicarbonate and phenol red, powder, suitable for cell culture

Synonym(s):

DME/Nutrient Mixture F-12 Ham, DMEM Hams F12, DME/F-12, 1:1 mixture

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About This Item

UNSPSC Code:

12352207

NACRES:

NA.75

MDL number:

MFCD00217417

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Product Name

Dulbecco′s Modified Eagle′s Medium/Nutrient Mixture F-12 Ham, With L-glutamine and 15 mM HEPES, without sodium bicarbonate and phenol red, powder, suitable for cell culture

Quality Level

500

form

powder

technique(s)

cell culture | mammalian: suitable

components

NaHCO₃: no
L-glutamine: yes
phenol red: no
sodium pyruvate: 0.055 g/L
glucose: 3.15 g/L
HEPES: 15 mM

shipped in

ambient

storage temp.

2-8°C

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Related Categories

Cell Culture & Analysis

Cell Culture Media & Buffers

Classical Media & Buffers

DMEM/F12 Media

Molecular Biology & Functional Genomics

General description

Dulbecco′s Modified Eagle′s Medium/Nutrient Mixture F-12 Ham is suitable for the growth of a wide range of cell types in low serum conditions. The removal of phenol red is often preferred by investigators working with clonal and stem cell cultures.

Application

Dulbecco′s Modified Eagle′s Medium/Nutrient Mixture F-12 Ham has been used to culture:

Hep-2 cells
mouse fibroblast L‐cells
L‐ Wnt family member 3A (Wnt3a) cells
primary buffalo embryonic fibroblast (BEF)

Preparation Note

Formulated to contain 15.6 grams of powder per liter of medium.

Supplement with 1.2 g/L sodium bicarbonate.

Storage Class

11 - Combustible Solids

wgk

WGK 1

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Knocking out or pharmaceutical inhibition of fatty acid binding protein 4 (FABP4) alleviates osteoarthritis induced by high-fat diet in mice.

C Zhang et al.

Osteoarthritis and cartilage, 26(6), 824-833 (2018-03-20)

Adipokines play roles in the pathogenesis of osteoarthritis (OA). Fatty acid binding protein 4 (FABP4) is a novel adipokine that is closely associated with obesity and metabolic diseases. The aim of this study was to discover the potential role of

CRISPR/Cas9-induced disruption of gene expression in mouse embryonic brain and single neural stem cells in vivo.

Nereo Kalebic et al.

EMBO reports, 17(3), 338-348 (2016-01-14)

We have applied the CRISPR/Cas9 system in vivo to disrupt gene expression in neural stem cells in the developing mammalian brain. Two days after in utero electroporation of a single plasmid encoding Cas9 and an appropriate guide RNA (gRNA) into

Oncostatin M expression in the mouse uterus during early pregnancy promotes embryo implantation and decidualization.

Tao Fu et al.

FEBS letters, 593(15), 2040-2050 (2019-06-04)

Oncostatin M (OSM) is a member of the interleukin-6 (IL-6) family, which functions in embryo implantation and decidualization. The expression, function and regulation of Osm in mouse uteri during early pregnancy remain unknown. We show that Osm is mainly expressed

The human WNT5A isoforms display similar patterns of expression but distinct and overlapping activities in normal human osteoblasts.

Dristi Bhandari et al.

Journal of cellular biochemistry, 122(10), 1262-1276 (2021-05-14)

WNT5A activates noncanonical Wnt signaling pathways and has critical functions in early development, differentiation, and tissue homeostasis. Two major WNT5A protein isoforms, which in this study we term WNT5A-L(A) and WNT5A-S(B), have been identified that differ by 18 AA at

The mammalian decidual cell evolved from a cellular stress response.

Eric M Erkenbrack et al.

PLoS biology, 16(8), e2005594-e2005594 (2018-08-25)

Among animal species, cell types vary greatly in terms of number and kind. The number of cell types found within an organism differs considerably between species, and cell type diversity is a significant contributor to differences in organismal structure and

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Articles

Nutrient Mixture F-12 Ham Formulation

Protocols

Powdered Media Preparation Instructions

Powdered media and salt mixtures are extremely hygroscopic and should be protected from atmospheric moisture.

粉末培养基制备说明

粉末培养基及盐混合物极具吸湿性，应注意防潮。

Global Trade Item Number

SKU	GTIN
D2906-10L	04061833562666
D2906-10X1L	04061835518944

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