Skip to Content
Merck
CN

D3687

DirectLoad PCR 100 bp Low Ladder

ready-to-use marker for DNA electrophoresis

Synonym(s):

100bp ladder for gel electrophoresis, 100bp maker, DirectLoad marker, RTU DNA ladder, RTU DNA marker, agarose gel electrophoresis ladder, agarose gel electrophoresis marker

Sign In to View Organizational & Contract Pricing

Select a Size


About This Item

UNSPSC Code:
41105335
NACRES:
NA.31
Technical Service
Need help? Our team of experienced scientists is here for you.
Let Us Assist
Technical Service
Need help? Our team of experienced scientists is here for you.
Let Us Assist

grade

Molecular Biology

Quality Level

form

liquid

usage

75 uses

solubility

water: soluble

suitability

suitable for electrophoresis (DNA)

storage temp.

−20°C

General description

Sigma′s DirectLoad PCR 100 bp Low Ladder contains 10 dsDNA fragments from 100 − 1,000 bases, evenly spaced in 100 bp repeats. Ladder should be loaded in a single lane on an agarose gel.

Application

Suitable for size determination of PCR generated DNA fragments with either agarose or polyacrylamide gels.

Features and Benefits

  • Ready-to-load
  • Easy-to-use
  • Popular band sizes

Other Notes

For optimal resolution, the recommended agarose gel concentration is 2.0%.
Sigma′s DirectLoad PCR 100 bp Low Ladder is provided in a solution of 5% glycerol, 4.2 mM EDTA, 0.09% orange G, and 0.0125% xylene cyanol.

Legal Information

DirectLoad is a trademark of Sigma-Aldrich Co. LLC

Storage Class Code

10 - Combustible liquids

WGK

WGK 3

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable

Regulatory Information

常规特殊物品
This item has

Choose from one of the most recent versions:

Certificates of Analysis (COA)

Lot/Batch Number

Don't see the Right Version?

If you require a particular version, you can look up a specific certificate by the Lot or Batch number.

Already Own This Product?

Find documentation for the products that you have recently purchased in the Document Library.

Visit the Document Library

Articles

Choose the appropriate markers and ladders for nucleic acid size determination of samples separated by electrophoresis. Determine size of DNA, RNA and PCR-generated fragments using agarose or polyacrylamide gels.

Markers for gel electrophoresis aid size determination of DNA, PCR fragments, and RNA, staining well with common nucleic acid stains.

选择适合的标记物和分子量标准,用于测定电泳分离样品的核酸大小。通过琼脂糖或聚丙烯酰胺凝胶检测DNA、RNA和PCR生成片段的大小。

Protocols

JumpStart™ Taq DNA Polymerase is an antibody-inactivated, hot start enzyme.

Protocol using antibody mediated hot start polymerase. Method has short activation period (<1min), and results in higher yields and more specificity over standard PCR methods.

使用抗体介导热启动聚合酶的实验方案。一种具有短活化阶段的方法(<1min), and results in higher yields and more specificity over standard PCR methods.

Our team of scientists has experience in all areas of research including Life Science, Material Science, Chemical Synthesis, Chromatography, Analytical and many others.

Contact Technical Service