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D4138

Deoxyribonuclease II from porcine spleen

Name: Deoxyribonuclease II from porcine spleen
Brand: SIGMA

Type IV, lyophilized powder, 2,000-6,000 Kunitz units/mg protein (biuret)

Synonym(s):

DNase II, Deoxyribonucleate 3′-oligonucleotido-hydrolase

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About This Item

CAS Number:

9025-64-3

UNSPSC Code:

12352204

NACRES:

NA.54

EC Number:

232-801-8

MDL number:

MFCD00130919

EC Number:

3.1.22.1 (BRENDA, IUBMB)

Specific activity:

2,000-6,000 Kunitz units/mg protein (biuret)

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Properties

type

Type IV

Quality Segment

200

form

lyophilized powder

specific activity

2,000-6,000 Kunitz units/mg protein (biuret)

mol wt

45 kDa

composition

Protein, 60-80%

foreign activity

RNase ≤0.05%

storage temp.

−20°C

Description

Application

DNase II from Sigma has been used to treat transformed cells during the purification of β-lactamase. It has also been used for the preparation of adenoma tissue in a study that investigated the effect of somatoprim on growth hormone secretion in human adenoma cell cultures (hSA).

Deoxyribonuclease II from porcine spleen has been used in an immunohistological study of the immune system cells in paraffin-embedded tissues. Deoxyribonuclease II from porcine spleen has also been used in a study to investigate its reassociation with the lysosomal membrane.

Biochem/physiol Actions

The enzyme is a lysosomal acid hydrolase that hydrolyzes DNA to 3′-phosphoryl oligonucleotides in the absence of metal ions, under acidic conditions. It is a non-covalently linked α·β heterodimer of approximately 45,000 Da molecular mass.

Physical form

Contains sodium chloride

Other Notes

One Kunitz unit will produce a ΔA₂₆₀ of 0.001 per min per mL at pH 4.6 at 25°C; [Mg²⁺] = 0.83 mM

Storage Class

11 - Combustible Solids

wgk

WGK 3

flash_point_f

Not applicable

flash_point_c

Not applicable

Regulatory Information

低风险生物材料

动植物源性产品

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Global Trade Item Number

SKU	GTIN
D4138-20KU	04061833564073
D4138-80KU	04061832755441